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mod header;
mod query;
mod record;
mod record_buf;
use futures::{stream, Stream};
use noodles_bgzf as bgzf;
use noodles_core::Region;
use noodles_csi::BinningIndex;
use noodles_sam::{self as sam, alignment::RecordBuf};
use tokio::io::{self, AsyncRead, AsyncReadExt, AsyncSeek};
use self::{header::read_header, query::query, record::read_record, record_buf::read_record_buf};
use crate::{io::reader::resolve_region, Record, MAGIC_NUMBER};
/// An async BAM reader.
///
/// # Examples
///
/// ```no_run
/// # #[tokio::main]
/// # async fn main() -> std::io::Result<()> {
/// use futures::TryStreamExt;
/// use noodles_bam as bam;
/// use tokio::fs::File;
///
/// let mut reader = File::open("sample.bam").await.map(bam::r#async::io::Reader::new)?;
/// let _header = reader.read_header().await?;
///
/// let mut records = reader.records();
///
/// while let Some(_record) = records.try_next().await? {
/// // ...
/// }
/// # Ok(())
/// # }
/// ```
pub struct Reader<R> {
inner: R,
buf: Vec<u8>,
}
impl<R> Reader<R> {
/// Returns a reference to the underlying reader.
///
/// # Examples
///
/// ```
/// use noodles_bam as bam;
/// use tokio::io;
/// let reader = bam::r#async::io::Reader::from(io::empty());
/// let _inner = reader.get_ref();
/// ```
pub fn get_ref(&self) -> &R {
&self.inner
}
/// Returns a mutable reference to the underlying reader.
///
/// # Examples
///
/// ```
/// use noodles_bam as bam;
/// use tokio::io;
/// let mut reader = bam::r#async::io::Reader::from(io::empty());
/// let _inner = reader.get_mut();
/// ```
pub fn get_mut(&mut self) -> &mut R {
&mut self.inner
}
/// Returns the underlying reader.
///
/// # Examples
///
/// ```
/// use noodles_bam as bam;
/// use tokio::io;
/// let reader = bam::r#async::io::Reader::from(io::empty());
/// let _inner = reader.into_inner();
/// ```
pub fn into_inner(self) -> R {
self.inner
}
}
impl<R> Reader<R>
where
R: AsyncRead + Unpin,
{
/// Reads the SAM header.
///
/// This verifies the BAM magic number, reads and parses the raw SAM header, and reads the
/// binary reference sequences. If the SAM header has a reference sequence dictionary, it must
/// match the binary reference sequences; otherwise, the binary reference sequences are added
/// to the SAM header.
///
/// The position of the stream is expected to be at the start.
///
/// # Examples
///
/// ```no_run
/// # use std::io;
/// #
/// # #[tokio::main]
/// # async fn main() -> io::Result<()> {
/// use noodles_bam as bam;
/// use tokio::fs::File;
///
/// let mut reader = File::open("sample.bam").await.map(bam::r#async::io::Reader::new)?;
/// let header = reader.read_header().await?;
/// # Ok(())
/// # }
/// ```
pub async fn read_header(&mut self) -> io::Result<sam::Header> {
read_magic(&mut self.inner).await?;
read_header(&mut self.inner).await
}
/// Reads a record into an alignment record buffer.
///
/// The record block size (`bs`) is read from the underlying stream and `bs` bytes are read
/// into an internal buffer. This buffer is then used to decode fields into the given record.
///
/// The stream is expected to be directly after the reference sequences or at the start of
/// another record.
///
/// It is more ergonomic to read records using a stream (see [`Self::records`] and
/// [`Self::query`]), but using this method directly allows the reuse of a [`RecordBuf`].
///
/// If successful, the record block size is returned. If a block size of 0 is returned, the
/// stream reached EOF.
///
/// # Examples
///
/// ```no_run
/// # #[tokio::main]
/// # async fn main() -> std::io::Result<()> {
/// use noodles_bam as bam;
/// use noodles_sam::alignment::RecordBuf;
/// use tokio::fs::File;
///
/// let mut reader = File::open("sample.bam").await.map(bam::r#async::io::Reader::new)?;
/// let header = reader.read_header().await?;
///
/// let mut record = RecordBuf::default();
/// reader.read_record_buf(&header, &mut record).await?;
/// # Ok(())
/// # }
/// ```
pub async fn read_record_buf(
&mut self,
header: &sam::Header,
record: &mut RecordBuf,
) -> io::Result<usize> {
read_record_buf(&mut self.inner, header, &mut self.buf, record).await
}
/// Reads a record.
///
/// The record block size (`bs`) is read from the underlying stream and `bs` bytes are read
/// into the record's buffer. No fields are decoded, meaning the record is not necessarily
/// valid. However, the structure of the buffer is guaranteed to be record-like.
///
/// The stream is expected to be directly after the reference sequences or at the start of
/// another record.
///
/// If successful, the record block size is returned. If a block size of 0 is returned, the
/// stream reached EOF.
///
/// # Examples
///
/// ```no_run
/// # use std::io;
/// #
/// # #[tokio::main]
/// # async fn main() -> io::Result<()> {
/// use noodles_bam as bam;
/// use tokio::fs::File;
///
/// let mut reader = File::open("sample.bam").await.map(bam::r#async::io::Reader::new)?;
/// reader.read_header().await?;
///
/// let mut record = bam::Record::default();
/// reader.read_record(&mut record).await?;
/// # Ok(())
/// # }
/// ```
pub async fn read_record(&mut self, record: &mut Record) -> io::Result<usize> {
let fields = record.fields_mut();
let block_size = match read_record(&mut self.inner, &mut fields.buf).await? {
0 => return Ok(0),
n => n,
};
fields.index()?;
Ok(block_size)
}
/// Returns an (async) stream over alignment record buffers starting from the current (input)
/// stream position.
///
/// The (input) stream is expected to be directly after the reference sequences or at the start
/// of another record.
///
/// # Examples
///
/// ```no_run
/// # #[tokio::main]
/// # async fn main() -> std::io::Result<()> {
/// use futures::TryStreamExt;
/// use noodles_bam as bam;
/// use tokio::fs::File;
///
/// let mut reader = File::open("sample.bam").await.map(bam::r#async::io::Reader::new)?;
/// let header = reader.read_header().await?;
///
/// let mut records = reader.record_bufs(&header);
///
/// while let Some(record) = records.try_next().await? {
/// // ...
/// }
/// # Ok(())
/// # }
/// ```
pub fn record_bufs<'a>(
&'a mut self,
header: &'a sam::Header,
) -> impl Stream<Item = io::Result<RecordBuf>> + '_ {
Box::pin(stream::try_unfold(
(&mut self.inner, &mut self.buf, RecordBuf::default()),
move |(reader, buf, mut record)| async move {
read_record_buf(reader, header, buf, &mut record)
.await
.map(|n| match n {
0 => None,
_ => Some((record.clone(), (reader, buf, record))),
})
},
))
}
/// Returns a stream over records.
///
/// The (input) stream is expected to be directly after the reference sequences or at the start
/// of another record.
///
/// # Examples
///
/// ```no_run
/// # use std::io;
/// #
/// # #[tokio::main]
/// # async fn main() -> io::Result<()> {
/// use futures::TryStreamExt;
/// use noodles_bam as bam;
/// use tokio::fs::File;
///
/// let mut reader = File::open("sample.bam").await.map(bam::r#async::io::Reader::new)?;
/// reader.read_header().await?;
///
/// let mut records = reader.records();
///
/// while let Some(record) = records.try_next().await? {
/// // ...
/// }
/// # Ok(())
/// # }
/// ```
pub fn records(&mut self) -> impl Stream<Item = io::Result<Record>> + '_ {
Box::pin(stream::try_unfold(
(self, Record::default()),
|(this, mut record)| async {
this.read_record(&mut record).await.map(|n| match n {
0 => None,
_ => Some((record.clone(), (this, record))),
})
},
))
}
}
impl<R> Reader<bgzf::AsyncReader<R>>
where
R: AsyncRead + Unpin,
{
/// Creates an async BAM reader.
///
/// The given reader must be a raw BGZF stream, as the underlying reader wraps it in a decoder.
///
/// # Examples
///
/// ```
/// use noodles_bam as bam;
/// let data = [];
/// let reader = bam::r#async::io::Reader::new(&data[..]);
/// ```
pub fn new(reader: R) -> Self {
Self::from(bgzf::AsyncReader::new(reader))
}
}
impl<R> Reader<bgzf::AsyncReader<R>>
where
R: AsyncRead + AsyncSeek + Unpin,
{
/// Returns a stream over records that intersect the given region.
///
/// # Examples
///
/// ```no_run
/// # #[tokio::main]
/// # async fn main() -> Result<(), Box<dyn std::error::Error>> {
/// use futures::TryStreamExt;
/// use noodles_bam::{self as bam, bai};
/// use noodles_core::Region;
/// use noodles_sam as sam;
/// use tokio::fs::File;
///
/// let mut reader = File::open("sample.bam").await.map(bam::AsyncReader::new)?;
/// let header = reader.read_header().await?;
///
/// let index = bai::r#async::read("sample.bam.bai").await?;
/// let region = "sq0:8-13".parse()?;
/// let mut query = reader.query(&header, &index, ®ion)?;
///
/// while let Some(record) = query.try_next().await? {
/// // ...
/// }
/// # Ok(())
/// # }
/// ```
pub fn query<I>(
&mut self,
header: &sam::Header,
index: &I,
region: &Region,
) -> io::Result<impl Stream<Item = io::Result<Record>> + '_>
where
I: BinningIndex,
{
let reference_sequence_id = resolve_region(header.reference_sequences(), region)?;
let chunks = index.query(reference_sequence_id, region.interval())?;
Ok(query(
self,
chunks,
reference_sequence_id,
region.interval(),
))
}
}
impl<R> From<R> for Reader<R> {
fn from(inner: R) -> Self {
Self {
inner,
buf: Vec::new(),
}
}
}
async fn read_magic<R>(reader: &mut R) -> io::Result<()>
where
R: AsyncRead + Unpin,
{
let mut magic = [0; 4];
reader.read_exact(&mut magic).await?;
if magic == MAGIC_NUMBER {
Ok(())
} else {
Err(io::Error::new(
io::ErrorKind::InvalidData,
"invalid BAM header",
))
}
}
#[cfg(test)]
mod tests {
use super::*;
#[tokio::test]
async fn test_read_magic() {
let data = b"BAM\x01";
let mut reader = &data[..];
assert!(read_magic(&mut reader).await.is_ok());
let data = [];
let mut reader = &data[..];
assert!(matches!(
read_magic(&mut reader).await,
Err(ref e) if e.kind() == io::ErrorKind::UnexpectedEof
));
let data = b"MThd";
let mut reader = &data[..];
assert!(matches!(
read_magic(&mut reader).await,
Err(ref e) if e.kind() == io::ErrorKind::InvalidData
));
}
}