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//! BAM reader.
mod builder;
pub(crate) mod header;
pub(crate) mod query;
mod record;
mod record_buf;
mod record_bufs;
mod records;
use std::{
ffi::CStr,
io::{self, Read},
};
use bstr::BString;
use noodles_bgzf as bgzf;
use noodles_core::Region;
use noodles_csi::BinningIndex;
use noodles_sam::{self as sam, alignment::RecordBuf, header::ReferenceSequences};
pub use self::{builder::Builder, query::Query, record_bufs::RecordBufs, records::Records};
use self::{record::read_record, record_buf::read_record_buf};
use crate::Record;
/// A BAM reader.
///
/// The BAM format is an encoded and compressed version of a SAM format.
///
/// The reader reads records sequentially but can use virtual positions to seek to offsets from the
/// start of a seekable stream.
///
/// # Examples
///
/// ```no_run
/// # use std::{fs::File, io};
/// use noodles_bam as bam;
///
/// let mut reader = File::open("sample.bam").map(bam::io::Reader::new)?;
/// let header = reader.read_header()?;
///
/// for result in reader.records() {
/// let record = result?;
/// // ...
/// }
/// # Ok::<_, io::Error>(())
/// ```
pub struct Reader<R> {
inner: R,
buf: Vec<u8>,
}
impl<R> Reader<R> {
/// Returns a reference to the underlying reader.
///
/// # Examples
///
/// ```
/// # use std::io;
/// use noodles_bam as bam;
/// let reader = bam::io::Reader::from(io::empty());
/// let _inner = reader.get_ref();
/// ```
pub fn get_ref(&self) -> &R {
&self.inner
}
/// Returns a mutable reference to the underlying reader.
///
/// # Examples
///
/// ```
/// # use std::io;
/// use noodles_bam as bam;
/// let mut reader = bam::io::Reader::from(io::empty());
/// let _inner = reader.get_mut();
/// ```
pub fn get_mut(&mut self) -> &mut R {
&mut self.inner
}
/// Returns the underlying reader.
///
/// # Examples
///
/// ```
/// # use std::io;
/// use noodles_bam as bam;
/// let reader = bam::io::Reader::from(io::empty());
/// let _inner = reader.into_inner();
/// ```
pub fn into_inner(self) -> R {
self.inner
}
}
impl<R> Reader<R>
where
R: Read,
{
/// Reads the SAM header.
///
/// This verifies the BAM magic number, reads and parses the raw SAM header, and reads the
/// binary reference sequences. If the SAM header has a reference sequence dictionary, it must
/// match the binary reference sequences; otherwise, the binary reference sequences are added
/// to the SAM header.
///
/// The position of the stream is expected to be at the start.
///
/// # Examples
///
/// ```no_run
/// # use std::{fs::File, io};
/// use noodles_bam as bam;
/// let mut reader = File::open("sample.bam").map(bam::io::Reader::new)?;
/// let header = reader.read_header()?;
/// # Ok::<(), io::Error>(())
/// ```
pub fn read_header(&mut self) -> io::Result<sam::Header> {
use self::header::read_header;
read_header(&mut self.inner)
}
/// Reads a record into an alignment record buffer.
///
/// The record block size (`bs`) is read from the underlying stream and `bs` bytes are read
/// into an internal buffer. This buffer is then used to decode fields into the given record.
///
/// The stream is expected to be directly after the reference sequences or at the start of
/// another record.
///
/// It is more ergonomic to read records using an iterator (see [`Self::records`] and
/// [`Self::query`]), but using this method directly allows the reuse of a single [`RecordBuf`]
/// buffer.
///
/// If successful, the record block size is returned. If a block size of 0 is returned, the
/// stream reached EOF.
///
/// # Examples
///
/// ```no_run
/// # use std::{fs::File, io};
/// use noodles_bam as bam;
/// use noodles_sam::alignment::RecordBuf;
///
/// let mut reader = File::open("sample.bam").map(bam::io::Reader::new)?;
/// let header = reader.read_header()?;
///
/// let mut record = RecordBuf::default();
/// reader.read_record_buf(&header, &mut record)?;
///
/// # Ok::<_, io::Error>(())
/// ```
pub fn read_record_buf(
&mut self,
header: &sam::Header,
record: &mut RecordBuf,
) -> io::Result<usize> {
read_record_buf(&mut self.inner, header, &mut self.buf, record)
}
/// Reads a record.
///
/// The record block size (`bs`) is read from the underlying stream and `bs` bytes are read
/// into the record's buffer. No fields are decoded, meaning the record is not necessarily
/// valid. However, the structure of the buffer is guaranteed to be record-like.
///
/// The stream is expected to be directly after the reference sequences or at the start of
/// another record.
///
/// If successful, the record block size is returned. If a block size of 0 is returned, the
/// stream reached EOF.
///
/// # Examples
///
/// ```no_run
/// # use std::{fs::File, io};
/// use noodles_bam as bam;
///
/// let mut reader = File::open("sample.bam").map(bam::io::Reader::new)?;
/// reader.read_header()?;
///
/// let mut record = bam::Record::default();
/// reader.read_record(&mut record)?;
/// # Ok::<(), io::Error>(())
/// ```
pub fn read_record(&mut self, record: &mut Record) -> io::Result<usize> {
let fields = record.fields_mut();
let block_size = match read_record(&mut self.inner, &mut fields.buf)? {
0 => return Ok(0),
n => n,
};
fields.index()?;
Ok(block_size)
}
/// Returns an iterator over alignment record buffers starting from the current stream
/// position.
///
/// The stream is expected to be directly after the reference sequences or at the start of
/// another record.
///
/// # Examples
///
/// ```no_run
/// # use std::{fs::File, io};
/// use noodles_bam as bam;
///
/// let mut reader = File::open("sample.bam").map(bam::io::Reader::new)?;
/// let header = reader.read_header()?;
///
/// for result in reader.record_bufs(&header) {
/// let record = result?;
/// println!("{:?}", record);
/// }
/// # Ok::<_, io::Error>(())
/// ```
pub fn record_bufs<'a>(&'a mut self, header: &'a sam::Header) -> RecordBufs<'_, R> {
RecordBufs::new(self, header)
}
/// Returns an iterator over records.
///
/// The stream is expected to be directly after the reference sequences or at the start of
/// another record.
///
/// # Examples
///
/// ```no_run
/// # use std::{fs::File, io};
/// use noodles_bam as bam;
///
/// let mut reader = File::open("sample.bam").map(bam::io::Reader::new)?;
/// reader.read_header()?;
///
/// for result in reader.records() {
/// let record = result?;
/// // ...
/// }
/// # Ok::<(), io::Error>(())
/// ```
pub fn records(&mut self) -> Records<'_, R> {
Records::new(self)
}
}
impl<R> Reader<bgzf::Reader<R>>
where
R: Read,
{
/// Creates a BAM reader.
///
/// The given reader must be a raw BGZF stream, as the underlying reader wraps it in a decoder.
///
/// # Examples
///
/// ```
/// use noodles_bam as bam;
/// let data = [];
/// let reader = bam::io::Reader::new(&data[..]);
/// ```
pub fn new(reader: R) -> Self {
Self::from(bgzf::Reader::new(reader))
}
}
impl<R> Reader<R>
where
R: bgzf::io::BufRead + bgzf::io::Seek,
{
// Seeks to the first record by setting the cursor to the beginning of the stream and
// (re)reading the header.
fn seek_to_first_record(&mut self) -> io::Result<bgzf::VirtualPosition> {
self.get_mut()
.seek_to_virtual_position(bgzf::VirtualPosition::default())?;
self.read_header()?;
Ok(self.get_ref().virtual_position())
}
/// Returns an iterator over records that intersect the given region.
///
/// To query for unmapped records, use [`Self::query_unmapped`].
///
/// # Examples
///
/// ```no_run
/// # use std::fs::File;
/// use noodles_bam::{self as bam, bai};
///
/// let mut reader = File::open("sample.bam").map(bam::io::Reader::new)?;
/// let header = reader.read_header()?;
///
/// let index = bai::read("sample.bam.bai")?;
/// let region = "sq0:8-13".parse()?;
/// let query = reader.query(&header, &index, ®ion)?;
///
/// for result in query {
/// let record = result?;
/// // ...
/// }
/// # Ok::<_, Box<dyn std::error::Error>>(())
/// ```
pub fn query<I>(
&mut self,
header: &sam::Header,
index: &I,
region: &Region,
) -> io::Result<Query<'_, R>>
where
I: BinningIndex,
{
let reference_sequence_id = resolve_region(header.reference_sequences(), region)?;
let chunks = index.query(reference_sequence_id, region.interval())?;
Ok(Query::new(
self.get_mut(),
chunks,
reference_sequence_id,
region.interval(),
))
}
/// Returns an iterator of unmapped records after querying for the unmapped region.
///
/// # Examples
///
/// ```no_run
/// # use std::{fs::File, io};
/// use noodles_bam::{self as bam, bai};
///
/// let mut reader = File::open("sample.bam").map(bam::io::Reader::new)?;
/// reader.read_header()?;
///
/// let index = bai::read("sample.bam.bai")?;
/// let query = reader.query_unmapped(&index)?;
///
/// for result in query {
/// let record = result?;
/// // ...
/// }
/// # Ok::<(), io::Error>(())
/// ```
pub fn query_unmapped<'r, I>(
&'r mut self,
index: &I,
) -> io::Result<impl Iterator<Item = io::Result<Record>> + 'r>
where
I: BinningIndex,
{
if let Some(pos) = index.last_first_record_start_position() {
self.get_mut().seek_to_virtual_position(pos)?;
} else {
self.seek_to_first_record()?;
}
Ok(self.records().filter(|result| {
result
.as_ref()
.map(|record| record.flags().is_unmapped())
.unwrap_or(true)
}))
}
}
impl<R> From<R> for Reader<R> {
fn from(inner: R) -> Self {
Self {
inner,
buf: Vec::new(),
}
}
}
impl<R> sam::alignment::io::Read<R> for Reader<R>
where
R: Read,
{
fn read_alignment_header(&mut self) -> io::Result<sam::Header> {
self.read_header()
}
fn alignment_records<'a>(
&'a mut self,
header: &'a sam::Header,
) -> Box<dyn Iterator<Item = io::Result<Box<dyn sam::alignment::Record>>> + 'a> {
Box::new(
self.record_bufs(header).map(|result| {
result.map(|record| Box::new(record) as Box<dyn sam::alignment::Record>)
}),
)
}
}
pub(crate) fn bytes_with_nul_to_bstring(buf: &[u8]) -> io::Result<BString> {
CStr::from_bytes_with_nul(buf)
.map(|c_str| c_str.to_bytes().into())
.map_err(|e| io::Error::new(io::ErrorKind::InvalidData, e))
}
pub(crate) fn resolve_region(
reference_sequences: &ReferenceSequences,
region: &Region,
) -> io::Result<usize> {
reference_sequences
.get_index_of(region.name())
.ok_or_else(|| {
io::Error::new(
io::ErrorKind::InvalidInput,
format!(
"region reference sequence does not exist in reference sequences: {region:?}"
),
)
})
}