1
  2
  3
  4
  5
  6
  7
  8
  9
 10
 11
 12
 13
 14
 15
 16
 17
 18
 19
 20
 21
 22
 23
 24
 25
 26
 27
 28
 29
 30
 31
 32
 33
 34
 35
 36
 37
 38
 39
 40
 41
 42
 43
 44
 45
 46
 47
 48
 49
 50
 51
 52
 53
 54
 55
 56
 57
 58
 59
 60
 61
 62
 63
 64
 65
 66
 67
 68
 69
 70
 71
 72
 73
 74
 75
 76
 77
 78
 79
 80
 81
 82
 83
 84
 85
 86
 87
 88
 89
 90
 91
 92
 93
 94
 95
 96
 97
 98
 99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
use std::{io, str};

use noodles_bam::record::ReferenceSequenceId;
use noodles_fasta as fasta;
use noodles_sam::{
    self as sam,
    record::{Data, QualityScores, Sequence},
};

use crate::data_container::CompressionHeader;

use super::{
    resolve::{resolve_bases, resolve_features},
    Record, Tag,
};

impl Record {
    /// Converts this CRAM record to a SAM record.
    pub fn try_into_sam_record(
        &self,
        reference_assembly: &[fasta::Record],
        reference_sequences: &sam::header::ReferenceSequences,
        compression_header: &CompressionHeader,
    ) -> io::Result<sam::Record> {
        let mut builder = sam::Record::builder();

        let raw_read_name = str::from_utf8(self.read_name())
            .map_err(|e| io::Error::new(io::ErrorKind::InvalidInput, e))?;

        if raw_read_name != "*" {
            let read_name = raw_read_name
                .parse()
                .map_err(|e| io::Error::new(io::ErrorKind::InvalidInput, e))?;

            builder = builder.set_read_name(read_name);
        }

        builder = builder.set_flags(self.bam_flags());

        if let Some(reference_sequence_name) =
            get_reference_sequence_name(reference_sequences, self.reference_sequence_id())?
        {
            builder = builder.set_reference_sequence_name(reference_sequence_name);
        }

        if let Some(alignment_start) = self.alignment_start() {
            builder = builder.set_position(alignment_start);
        }

        if let Some(mapping_quality) = self.mapping_quality() {
            builder = builder.set_mapping_quality(mapping_quality);
        }

        let cigar = resolve_features(self.features(), self.read_length() as i32);
        builder = builder.set_cigar(cigar);

        if let Some(mate_reference_sequence_name) = get_reference_sequence_name(
            reference_sequences,
            self.next_fragment_reference_sequence_id(),
        )? {
            builder = builder.set_mate_reference_sequence_name(mate_reference_sequence_name);
        }

        if let Some(next_mate_alignment_start) = self.next_mate_alignment_start() {
            builder = builder.set_mate_position(next_mate_alignment_start);
        }

        builder = builder.set_template_length(self.template_size());

        if self.read_length() > 0 {
            let sequence = if let Some(reference_sequence_id) = self.reference_sequence_id() {
                let reference_sequence_record = usize::try_from(i32::from(reference_sequence_id))
                    .map_err(|e| io::Error::new(io::ErrorKind::InvalidData, e))
                    .and_then(|id| {
                        reference_assembly.get(id).ok_or_else(|| {
                            io::Error::new(io::ErrorKind::InvalidData, "missing reference sequence")
                        })
                    })?;

                let raw_bases = resolve_bases(
                    reference_sequence_record,
                    compression_header,
                    self.features(),
                    self.alignment_start().map(i32::from).unwrap_or_default(),
                    self.read_length() as usize,
                );

                bytes_to_sequence(&raw_bases)?
            } else {
                bytes_to_sequence(self.bases())?
            };

            builder = builder.set_sequence(sequence);
        }

        if !self.quality_scores().is_empty() {
            let quality_scores = bytes_to_quality_scores(self.quality_scores())?;
            builder = builder.set_quality_scores(quality_scores);
        }

        if !self.tags().is_empty() {
            let data = tags_to_data(self.tags())?;
            builder = builder.set_data(data);
        }

        builder
            .build()
            .map_err(|e| io::Error::new(io::ErrorKind::InvalidData, e))
    }
}

fn get_reference_sequence_name(
    reference_sequences: &sam::header::ReferenceSequences,
    reference_sequence_id: Option<ReferenceSequenceId>,
) -> io::Result<Option<sam::record::ReferenceSequenceName>> {
    reference_sequence_id
        .map(i32::from)
        .map(|id| {
            reference_sequences
                .get_index(id as usize)
                .and_then(|(_, rs)| rs.name().parse().ok())
                .ok_or_else(|| {
                    io::Error::new(io::ErrorKind::InvalidInput, "invalid reference sequence ID")
                })
        })
        .transpose()
}

fn bytes_to_sequence(data: &[u8]) -> io::Result<Sequence> {
    use sam::record::sequence::Base;

    data.iter()
        .copied()
        .map(|b| Base::try_from(b as char))
        .collect::<Result<Vec<_>, _>>()
        .map_err(|e| io::Error::new(io::ErrorKind::InvalidInput, e))
        .map(Sequence::from)
}

fn bytes_to_quality_scores(data: &[u8]) -> io::Result<QualityScores> {
    use sam::record::quality_scores::Score;

    data.iter()
        .copied()
        .map(Score::try_from)
        .collect::<Result<Vec<_>, _>>()
        .map_err(|e| io::Error::new(io::ErrorKind::InvalidInput, e))
        .map(QualityScores::from)
}

fn tags_to_data(tags: &[Tag]) -> io::Result<Data> {
    use sam::record::data::Field;

    let mut fields = Vec::with_capacity(tags.len());

    for tag in tags {
        let raw_tag = tag.key().tag();
        let sam_tag = str::from_utf8(&raw_tag)
            .map_err(|e| io::Error::new(io::ErrorKind::InvalidInput, e))
            .and_then(|s| {
                s.parse()
                    .map_err(|e| io::Error::new(io::ErrorKind::InvalidInput, e))
            })?;

        let bam_value = tag.value();
        let sam_value = bam_value.clone().into();

        let field = Field::new(sam_tag, sam_value);
        fields.push(field);
    }

    Data::try_from(fields).map_err(|e| io::Error::new(io::ErrorKind::InvalidInput, e))
}