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//! CRAM reader and record iterator.
mod builder;
pub(crate) mod container;
pub(crate) mod data_container;
pub(crate) mod header_container;
pub(crate) mod num;
mod query;
pub(crate) mod record;
mod records;
pub use self::{builder::Builder, query::Query, records::Records};
use std::io::{self, Read, Seek, SeekFrom};
use bytes::BytesMut;
use noodles_core::Region;
use noodles_fasta as fasta;
use noodles_sam as sam;
use crate::{
crai, data_container::DataContainer, file_definition::Version, FileDefinition, MAGIC_NUMBER,
};
/// A CRAM reader.
///
/// The CRAM format is comprised of four main parts: 1) a file definition, 2) a file header, 3) a
/// list of data containers, and 4) an end-of-file (EOF) container.
///
/// # Examples
///
/// ```no_run
/// # use std::{fs::File, io};
/// use noodles_cram as cram;
/// use noodles_fasta as fasta;
///
/// let mut reader = File::open("sample.cram").map(cram::io::Reader::new)?;
/// let header = reader.read_header()?;
///
/// for result in reader.records(&header) {
/// let record = result?;
/// println!("{:?}", record);
/// }
///
/// # Ok::<_, io::Error>(())
/// ```
pub struct Reader<R> {
inner: R,
reference_sequence_repository: fasta::Repository,
buf: BytesMut,
}
impl<R> Reader<R>
where
R: Read,
{
/// Creates a CRAM reader.
///
/// # Examples
///
/// ```no_run
/// # use std::{fs::File, io};
/// use noodles_cram as cram;
/// let mut reader = File::open("sample.bam").map(cram::io::Reader::new)?;
/// # Ok::<(), io::Error>(())
/// ```
pub fn new(inner: R) -> Self {
Builder::default().build_from_reader(inner)
}
/// Returns a reference to the underlying reader.
///
/// # Examples
///
/// ```
/// use noodles_cram as cram;
/// let data = [];
/// let reader = cram::io::Reader::new(&data[..]);
/// assert!(reader.get_ref().is_empty());
/// ```
pub fn get_ref(&self) -> &R {
&self.inner
}
/// Returns a mutable reference to the underlying reader.
///
/// # Examples
///
/// ```
/// use noodles_cram as cram;
/// let data = [];
/// let mut reader = cram::io::Reader::new(&data[..]);
/// assert!(reader.get_mut().is_empty());
/// ```
pub fn get_mut(&mut self) -> &mut R {
&mut self.inner
}
/// Unwraps and returns the underlying reader.
///
/// # Examples
///
/// ```
/// use noodles_cram as cram;
/// let data = [];
/// let reader = cram::io::Reader::new(&data[..]);
/// assert!(reader.into_inner().is_empty());
/// ```
pub fn into_inner(self) -> R {
self.inner
}
pub(crate) fn reference_sequence_repository(&self) -> &fasta::Repository {
&self.reference_sequence_repository
}
/// Reads the CRAM file definition.
///
/// The CRAM magic number is also checked.
///
/// The position of the stream is expected to be at the start.
///
/// # Examples
///
/// ```no_run
/// # use std::{fs::File, io};
/// use noodles_cram as cram;
/// let mut reader = File::open("sample.cram").map(cram::io::Reader::new)?;
/// let file_definition = reader.read_file_definition()?;
/// # Ok::<(), io::Error>(())
/// ```
pub fn read_file_definition(&mut self) -> io::Result<FileDefinition> {
read_magic_number(&mut self.inner)?;
let format = read_format(&mut self.inner)?;
let file_id = read_file_id(&mut self.inner)?;
Ok(FileDefinition::new(format, file_id))
}
/// Reads the SAM header.
///
/// The position of the stream is expected to be at the CRAM header container, i.e., directly
/// after the file definition.
///
/// # Examples
///
/// ```no_run
/// # use std::{fs::File, io};
/// use noodles_cram as cram;
///
/// let mut reader = File::open("sample.cram").map(cram::io::Reader::new)?;
/// reader.read_file_definition()?;
///
/// let header = reader.read_file_header()?;
/// # Ok::<(), io::Error>(())
/// ```
pub fn read_file_header(&mut self) -> io::Result<sam::Header> {
use self::header_container::read_header_container;
read_header_container(&mut self.inner, &mut self.buf)
}
/// Reads the SAM header.
///
/// This verifies the CRAM magic number, discards the file definition, and reads and parses the
/// file header as a SAM header.
///
/// The position of the stream is expected to be at the start.
///
/// # Examples
///
/// ```no_run
/// # use std::{fs::File, io};
/// use noodles_cram as cram;
/// let mut reader = File::open("sample.cram").map(cram::io::Reader::new)?;
/// let header = reader.read_header()?;
/// # Ok::<(), io::Error>(())
/// ```
pub fn read_header(&mut self) -> io::Result<sam::Header> {
self.read_file_definition()?;
self.read_file_header()
}
pub(crate) fn read_data_container_with_container_header(
&mut self,
) -> io::Result<Option<(crate::data_container::Header, DataContainer)>> {
use self::data_container::read_data_container_with_container_header;
read_data_container_with_container_header(&mut self.inner, &mut self.buf)
}
/// Reads a data container.
///
/// This returns `None` if the container header is the EOF container header, which signals the
/// end of the stream.
///
/// # Examples
///
/// ```no_run
/// # use std::{fs::File, io};
/// use noodles_cram as cram;
///
/// let mut reader = File::open("sample.cram").map(cram::io::Reader::new)?;
/// reader.read_header()?;
///
/// while let Some(container) = reader.read_data_container()? {
/// // ...
/// }
/// # Ok::<(), io::Error>(())
/// ```
pub fn read_data_container(&mut self) -> io::Result<Option<DataContainer>> {
use self::data_container::read_data_container;
read_data_container(&mut self.inner, &mut self.buf)
}
/// Returns a iterator over records starting from the current stream position.
///
/// The stream is expected to be at the start of a data container.
///
/// # Examples
///
/// ```no_run
/// # use std::{fs::File, io};
/// use noodles_cram as cram;
/// use noodles_fasta as fasta;
///
/// let mut reader = File::open("sample.cram").map(cram::io::Reader::new)?;
/// let header = reader.read_header()?;
///
/// for result in reader.records(&header) {
/// let record = result?;
/// // ...
/// }
/// # Ok::<_, io::Error>(())
/// ```
pub fn records<'r>(&'r mut self, header: &'r sam::Header) -> Records<'r, R> {
Records::new(self, header)
}
}
impl<R> Reader<R>
where
R: Read + Seek,
{
/// Seeks the underlying reader to the given position.
///
/// Positions typically come from the associated CRAM index file.
///
/// # Examples
///
/// ```no_run
/// # use std::{fs::File, io};
/// use std::io::SeekFrom;
/// use noodles_cram as cram;
///
/// let mut reader = File::open("sample.cram").map(cram::io::Reader::new)?;
/// reader.seek(SeekFrom::Start(17711))?;
/// # Ok::<(), io::Error>(())
/// ```
pub fn seek(&mut self, pos: SeekFrom) -> io::Result<u64> {
self.inner.seek(pos)
}
/// Returns the current position of the underlying reader.
///
/// # Examples
///
/// ```
/// # use std::io::{self, Cursor};
/// use noodles_cram as cram;
/// let data = Cursor::new(Vec::new());
/// let mut reader = cram::io::Reader::new(data);
/// let position = reader.position()?;
/// assert_eq!(position, 0);
/// # Ok::<(), io::Error>(())
/// ```
pub fn position(&mut self) -> io::Result<u64> {
self.inner.stream_position()
}
/// Returns an iterator over records that intersects the given region.
///
/// # Examples
///
/// ```no_run
/// # use std::{fs::File, io};
/// use noodles_cram::{self as cram, crai};
/// use noodles_fasta as fasta;
///
/// let mut reader = File::open("sample.cram").map(cram::io::Reader::new)?;
///
/// let header = reader.read_header()?;
/// let index = crai::read("sample.cram.crai")?;
/// let region = "sq0:8-13".parse()?;
/// let query = reader.query(&header, &index, ®ion)?;
///
/// for result in query {
/// let record = result?;
/// // ...
/// }
/// # Ok::<_, Box<dyn std::error::Error>>(())
/// ```
pub fn query<'a>(
&'a mut self,
header: &'a sam::Header,
index: &'a crai::Index,
region: &Region,
) -> io::Result<Query<'_, R>> {
let reference_sequence_id = header
.reference_sequences()
.get_index_of(region.name())
.ok_or_else(|| {
io::Error::new(
io::ErrorKind::InvalidInput,
"invalid reference sequence name",
)
})?;
Ok(Query::new(
self,
header,
index,
reference_sequence_id,
region.interval(),
))
}
}
impl<R> sam::alignment::io::Read<R> for Reader<R>
where
R: Read,
{
fn read_alignment_header(&mut self) -> io::Result<sam::Header> {
self.read_header()
}
fn alignment_records<'a>(
&'a mut self,
header: &'a sam::Header,
) -> Box<dyn Iterator<Item = io::Result<Box<dyn sam::alignment::Record>>> + 'a> {
Box::new(self.records(header).map(|result| {
result.and_then(|record| {
record
.try_into_alignment_record(header)
.map(|alignment_record| {
Box::new(alignment_record) as Box<dyn sam::alignment::Record>
})
})
}))
}
}
fn read_magic_number<R>(reader: &mut R) -> io::Result<()>
where
R: Read,
{
let mut buf = [0; 4];
reader.read_exact(&mut buf)?;
if buf == MAGIC_NUMBER {
Ok(())
} else {
Err(io::Error::new(
io::ErrorKind::InvalidData,
"invalid CRAM header",
))
}
}
fn read_format<R>(reader: &mut R) -> io::Result<Version>
where
R: Read,
{
let mut buf = [0; 2];
reader.read_exact(&mut buf)?;
Ok(Version::new(buf[0], buf[1]))
}
fn read_file_id<R>(reader: &mut R) -> io::Result<[u8; 20]>
where
R: Read,
{
let mut buf = [0; 20];
reader.read_exact(&mut buf)?;
Ok(buf)
}
#[cfg(test)]
mod tests {
use super::*;
#[test]
fn test_read_file_definition() -> Result<(), Box<dyn std::error::Error>> {
let data = [
0x43, 0x52, 0x41, 0x4d, // magic number = b"CRAM"
0x03, 0x00, // format version = (3, 0)
0x00, 0x68, 0xac, 0xf3, 0x06, 0x4d, 0xaa, 0x1e, 0x29, 0xa4, 0xa0, 0x8c, 0x56, 0xee,
0x91, 0x9b, 0x91, 0x04, 0x21, 0x1f, // file ID
];
let mut reader = Reader::new(&data[..]);
let actual = reader.read_file_definition()?;
let file_id = <[u8; 20]>::try_from(&data[6..])?;
let expected = FileDefinition::new(Version::new(3, 0), file_id);
assert_eq!(actual, expected);
Ok(())
}
#[test]
fn test_read_magic_number() {
let data = b"CRAM";
let mut reader = &data[..];
assert!(read_magic_number(&mut reader).is_ok());
}
#[test]
fn test_read_magic_number_with_invalid_input() {
let data = [];
let mut reader = &data[..];
assert!(matches!(
read_magic_number(&mut reader),
Err(ref e) if e.kind() == io::ErrorKind::UnexpectedEof,
));
let data = b"BAM\x01";
let mut reader = &data[..];
assert!(matches!(
read_magic_number(&mut reader),
Err(ref e) if e.kind() == io::ErrorKind::InvalidData,
));
}
}