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//! CRAM writer.
pub(crate) mod builder;
pub(crate) mod container;
pub(crate) mod data_container;
pub(crate) mod header_container;
pub(crate) mod num;
mod options;
pub(crate) mod record;
pub use self::builder::Builder;
pub(crate) use self::options::Options;
use std::{
io::{self, Write},
mem,
};
use noodles_fasta as fasta;
use noodles_sam::{self as sam, header::ReferenceSequences};
use crate::{file_definition::Version, DataContainer, FileDefinition, Record, MAGIC_NUMBER};
/// A CRAM writer.
///
/// A call to [`Self::try_finish`] must be made before the writer is dropped.
///
/// # Examples
///
/// ```
/// # use std::io;
/// use noodles_cram as cram;
/// use noodles_sam as sam;
///
/// let mut writer = cram::io::Writer::new(Vec::new());
///
/// let header = sam::Header::default();
/// writer.write_header(&header)?;
///
/// let record = cram::Record::default();
/// writer.write_record(&header, record)?;
///
/// writer.try_finish(&header)?;
/// # Ok::<(), io::Error>(())
/// ```
#[derive(Debug)]
pub struct Writer<W>
where
W: Write,
{
inner: W,
reference_sequence_repository: fasta::Repository,
options: Options,
data_container_builder: crate::data_container::Builder,
record_counter: u64,
}
impl<W> Writer<W>
where
W: Write,
{
/// Creates a new CRAM writer with default options.
///
/// # Examples
///
/// ```
/// use noodles_cram as cram;
/// let writer = cram::io::Writer::new(Vec::new());
/// ```
pub fn new(inner: W) -> Self {
Builder::default().build_with_writer(inner)
}
/// Returns a reference to the underlying writer.
///
/// # Examples
///
/// ```
/// use noodles_cram as cram;
/// let writer = cram::io::Writer::new(Vec::new());
/// assert!(writer.get_ref().is_empty());
/// ```
pub fn get_ref(&self) -> &W {
&self.inner
}
/// Attempts to finish the output stream by writing any pending containers and a final EOF
/// container.
///
/// This is typically only manually called if the underlying stream is needed before the writer
/// is dropped.
///
/// # Examples
///
/// ```
/// # use std::io;
/// use noodles_cram as cram;
/// use noodles_sam as sam;
///
/// let header = sam::Header::default();
/// let mut writer = cram::io::Writer::new(Vec::new());
/// writer.try_finish(&header)?;
/// # Ok::<(), io::Error>(())
/// ```
pub fn try_finish(&mut self, header: &sam::Header) -> io::Result<()> {
use self::container::write_eof_container;
self.flush(header)?;
write_eof_container(&mut self.inner)
}
/// Writes a CRAM file definition.
///
/// The file ID is set as a blank value (`[0x00; 20]`).
///
/// # Examples
///
/// ```
/// # use std::io;
/// use noodles_cram as cram;
///
/// let mut writer = cram::io::Writer::new(Vec::new());
/// writer.write_file_definition()?;
///
/// assert_eq!(writer.get_ref(), &[
/// // magic number (CRAM)
/// 0x43, 0x52, 0x41, 0x4d,
/// // format (major, minor)
/// 0x03, 0x00,
/// // file ID
/// 0x00, 0x00, 0x00, 0x00, 0x00, 0x00, 0x00, 0x00, 0x00, 0x00,
/// 0x00, 0x00, 0x00, 0x00, 0x00, 0x00, 0x00, 0x00, 0x00, 0x00,
/// ]);
/// # Ok::<(), io::Error>(())
/// ```
pub fn write_file_definition(&mut self) -> io::Result<()> {
let file_definition = FileDefinition::new(self.options.version, Default::default());
write_file_definition(&mut self.inner, &file_definition)
}
/// Writes a CRAM file header container.
///
/// The position of the stream is expected to be directly after the file definition.
///
/// Entries in the reference sequence dictionary that are missing MD5 checksums (`M5`) will
/// automatically be calculated and added to the written record.
///
/// # Examples
///
/// ```
/// # use std::io;
/// use noodles_cram as cram;
/// use noodles_sam as sam;
///
/// let mut writer = cram::io::Writer::new(Vec::new());
/// writer.write_file_definition()?;
///
/// let header = sam::Header::default();
/// writer.write_file_header(&header)?;
///
/// writer.try_finish(&header)?;
/// # Ok::<(), io::Error>(())
/// ```
pub fn write_file_header(&mut self, header: &sam::Header) -> io::Result<()> {
use self::header_container::write_header_container;
let mut header = header.clone();
add_missing_reference_sequence_checksums(
&self.reference_sequence_repository,
header.reference_sequences_mut(),
)?;
write_header_container(&mut self.inner, &header)
}
/// Writes a SAM header.
///
/// This writes the CRAM magic number, the file definition, and file header using the given SAM
/// header.
///
/// ```
/// # use std::io;
/// use noodles_cram as cram;
/// use noodles_sam as sam;
///
/// let mut writer = cram::io::Writer::new(io::sink());
///
/// let header = sam::Header::builder().add_comment("noodles-cram").build();
/// writer.write_header(&header)?;
/// # Ok::<_, io::Error>(())
/// ```
pub fn write_header(&mut self, header: &sam::Header) -> io::Result<()> {
self.write_file_definition()?;
self.write_file_header(header)
}
/// Writes a CRAM record.
///
/// # Examples
///
/// ```
/// # use std::io;
/// use noodles_cram as cram;
/// use noodles_sam as sam;
///
/// let mut writer = cram::io::Writer::new(Vec::new());
///
/// let header = sam::Header::default();
/// writer.write_header(&header)?;
///
/// let record = cram::Record::default();
/// writer.write_record(&header, record)?;
///
/// writer.try_finish(&header)?;
/// # Ok::<(), io::Error>(())
/// ```
pub fn write_record(&mut self, header: &sam::Header, mut record: Record) -> io::Result<()> {
use crate::data_container::builder::AddRecordError;
loop {
match self.data_container_builder.add_record(record) {
Ok(_) => {
self.record_counter += 1;
return Ok(());
}
Err(e) => match e {
AddRecordError::ContainerFull(r) => {
record = r;
self.flush(header)?;
}
AddRecordError::SliceFull(r) => {
record = r;
}
_ => return Err(io::Error::from(io::ErrorKind::InvalidInput)),
},
}
}
}
fn flush(&mut self, header: &sam::Header) -> io::Result<()> {
use self::data_container::write_data_container;
if self.data_container_builder.is_empty() {
return Ok(());
}
let data_container_builder = mem::replace(
&mut self.data_container_builder,
DataContainer::builder(self.record_counter),
);
let base_count = data_container_builder.base_count();
let data_container = data_container_builder.build(
&self.options,
&self.reference_sequence_repository,
header,
)?;
write_data_container(&mut self.inner, &data_container, base_count)
}
}
impl<W> sam::alignment::io::Write for Writer<W>
where
W: Write,
{
fn write_alignment_header(&mut self, header: &sam::Header) -> io::Result<()> {
self.write_header(header)
}
fn write_alignment_record(
&mut self,
header: &sam::Header,
record: &dyn sam::alignment::Record,
) -> io::Result<()> {
let r = Record::try_from_alignment_record(header, record)?;
self.write_record(header, r)
}
fn finish(&mut self, header: &sam::Header) -> io::Result<()> {
self.try_finish(header)
}
}
fn write_file_definition<W>(writer: &mut W, file_definition: &FileDefinition) -> io::Result<()>
where
W: Write,
{
writer.write_all(MAGIC_NUMBER)?;
write_format(writer, file_definition.version())?;
writer.write_all(file_definition.file_id())?;
Ok(())
}
fn write_format<W>(writer: &mut W, version: Version) -> io::Result<()>
where
W: Write,
{
let format = [version.major(), version.minor()];
writer.write_all(&format)
}
pub(crate) fn add_missing_reference_sequence_checksums(
reference_sequence_repository: &fasta::Repository,
reference_sequences: &mut ReferenceSequences,
) -> io::Result<()> {
use indexmap::map::Entry;
use sam::header::record::value::map::reference_sequence::{tag, Md5Checksum};
use crate::data_container::slice::builder::calculate_normalized_sequence_digest;
for (name, reference_sequence) in reference_sequences {
if let Entry::Vacant(entry) = reference_sequence
.other_fields_mut()
.entry(tag::MD5_CHECKSUM)
{
let sequence = reference_sequence_repository
.get(name)
.transpose()?
.expect("missing reference sequence");
let checksum = calculate_normalized_sequence_digest(&sequence[..]);
entry.insert(Md5Checksum::from(checksum).to_string().into());
}
}
Ok(())
}
#[cfg(test)]
mod tests {
use bstr::BString;
use super::*;
#[test]
fn test_add_missing_reference_sequence_checksums() -> Result<(), Box<dyn std::error::Error>> {
use std::num::NonZeroUsize;
use fasta::record::{Definition, Sequence};
use sam::header::record::value::{
map::{reference_sequence::tag, ReferenceSequence},
Map,
};
const SQ0_LN: NonZeroUsize = match NonZeroUsize::new(8) {
Some(length) => length,
None => unreachable!(),
};
const SQ1_LN: NonZeroUsize = match NonZeroUsize::new(13) {
Some(length) => length,
None => unreachable!(),
};
let reference_sequences = vec![
fasta::Record::new(
Definition::new("sq0", None),
Sequence::from(b"TTCACCCA".to_vec()),
),
fasta::Record::new(
Definition::new("sq1", None),
Sequence::from(b"GATCTTACTTTTT".to_vec()),
),
];
let sq0_md5_checksum = BString::from("be19336b7e15968f7ac7dc82493d9cd8");
let sq1_md5_checksum = BString::from("d80f22a19aeeb623b3e4f746c762f21d");
let repository = fasta::Repository::new(reference_sequences);
let mut header = sam::Header::builder()
.add_reference_sequence("sq0", Map::<ReferenceSequence>::new(SQ0_LN))
.add_reference_sequence(
"sq1",
Map::<ReferenceSequence>::builder()
.set_length(SQ1_LN)
.insert(tag::MD5_CHECKSUM, sq1_md5_checksum.clone())
.build()?,
)
.build();
add_missing_reference_sequence_checksums(&repository, header.reference_sequences_mut())?;
let sq0 = header.reference_sequences().get(&b"sq0"[..]);
assert_eq!(
sq0.and_then(|rs| rs.other_fields().get(&tag::MD5_CHECKSUM)),
Some(&sq0_md5_checksum)
);
let sq1 = header.reference_sequences().get(&b"sq1"[..]);
assert_eq!(
sq1.and_then(|rs| rs.other_fields().get(&tag::MD5_CHECKSUM)),
Some(&sq1_md5_checksum)
);
Ok(())
}
}