noodles_cram/async/io/writer.rs
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 257 258 259 260 261 262 263 264 265 266 267 268 269 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284 285 286 287 288 289 290 291 292 293 294 295 296 297 298 299 300 301 302 303 304 305 306 307 308 309 310 311 312
//! Async CRAM writer.
mod builder;
mod container;
mod data_container;
mod header_container;
use std::mem;
use noodles_fasta as fasta;
use noodles_sam as sam;
use tokio::io::{self, AsyncWrite, AsyncWriteExt};
pub use self::builder::Builder;
use crate::{
file_definition::Version, io::writer::Options, DataContainer, FileDefinition, Record,
MAGIC_NUMBER,
};
/// An async CRAM writer.
///
/// A call to [`Self::shutdown`] must be made before the writer is dropped.
pub struct Writer<W> {
inner: W,
reference_sequence_repository: fasta::Repository,
options: Options,
data_container_builder: crate::data_container::Builder,
record_counter: u64,
}
impl<W> Writer<W>
where
W: AsyncWrite + Unpin,
{
/// Creates an async CRAM writer.
///
/// # Examples
///
/// ```
/// use noodles_cram as cram;
/// use tokio::io;
/// let writer = cram::r#async::io::Writer::new(io::sink());
/// ```
pub fn new(inner: W) -> Self {
Builder::default().build_from_writer(inner)
}
/// Returns a reference to the underlying writer.
///
/// # Examples
///
/// ```
/// use noodles_cram as cram;
/// use tokio::io;
/// let writer = cram::r#async::io::Writer::new(io::sink());
/// let inner = writer.get_ref();
/// ```
pub fn get_ref(&self) -> &W {
&self.inner
}
/// Attempts to shutdown the output stream by writing any pending containers and a final EOF
/// container.
///
/// # Examples
///
/// ```
/// # #[tokio::main]
/// # async fn main() -> std::io::Result<()> {
/// use noodles_cram as cram;
/// use noodles_sam as sam;
/// use tokio::io;
/// let mut writer = cram::r#async::io::Writer::new(io::sink());
/// let header = sam::Header::default();
/// writer.shutdown(&header).await?;
/// # Ok(())
/// # }
/// ```
pub async fn shutdown(&mut self, header: &sam::Header) -> io::Result<()> {
use self::container::write_eof_container;
self.flush(header).await?;
write_eof_container(&mut self.inner).await
}
/// Writes a CRAM file definition.
///
/// The file ID is set as a blank value (`[0x00; 20]`).
///
/// # Examples
///
/// ```
/// # #[tokio::main]
/// # async fn main() -> std::io::Result<()> {
/// use noodles_cram as cram;
///
/// let mut writer = cram::r#async::io::Writer::new(Vec::new());
/// writer.write_file_definition().await?;
///
/// assert_eq!(writer.get_ref(), &[
/// // magic number (CRAM)
/// 0x43, 0x52, 0x41, 0x4d,
/// // format (major, minor)
/// 0x03, 0x00,
/// // file ID
/// 0x00, 0x00, 0x00, 0x00, 0x00, 0x00, 0x00, 0x00, 0x00, 0x00,
/// 0x00, 0x00, 0x00, 0x00, 0x00, 0x00, 0x00, 0x00, 0x00, 0x00,
/// ]);
/// # Ok(())
/// # }
/// ```
pub async fn write_file_definition(&mut self) -> io::Result<()> {
let file_definition = FileDefinition::default();
write_file_definition(&mut self.inner, &file_definition).await
}
/// Writes a CRAM file header container.
///
/// The position of the stream is expected to be directly after the file definition.
///
/// Entries in the reference sequence dictionary that are missing MD5 checksums (`M5`) will
/// automatically be calculated and added to the written record.
///
/// # Examples
///
/// ```
/// # #[tokio::main]
/// # async fn main() -> std::io::Result<()> {
/// use noodles_cram as cram;
/// use noodles_sam as sam;
/// use tokio::io;
///
/// let mut writer = cram::r#async::io::Writer::new(io::sink());
/// writer.write_file_definition().await?;
///
/// let header = sam::Header::default();
/// writer.write_file_header(&header).await?;
///
/// writer.shutdown(&header).await?;
/// # Ok(())
/// # }
/// ```
pub async fn write_file_header(&mut self, header: &sam::Header) -> io::Result<()> {
use self::header_container::write_header_container;
use crate::io::writer::add_missing_reference_sequence_checksums;
let mut header = header.clone();
add_missing_reference_sequence_checksums(
&self.reference_sequence_repository,
header.reference_sequences_mut(),
)?;
write_header_container(&mut self.inner, &header).await
}
/// Writes a SAM header.
///
/// This writes the CRAM magic number, the file definition, and file header using the given SAM
/// header.
///
/// ```
/// # #[tokio::main]
/// # async fn main() -> tokio::io::Result<()> {
/// use noodles_cram as cram;
/// use noodles_sam as sam;
/// use tokio::io;
///
/// let mut writer = cram::r#async::io::Writer::new(io::sink());
///
/// let header = sam::Header::default();
/// writer.write_header(&header).await?;
///
/// writer.shutdown(&header).await?;
/// # Ok(())
/// # }
/// ```
pub async fn write_header(&mut self, header: &sam::Header) -> io::Result<()> {
self.write_file_definition().await?;
self.write_file_header(header).await
}
/// Writes a CRAM record.
///
/// # Examples
///
/// ```
/// # #[tokio::main]
/// # async fn main() -> std::io::Result<()> {
/// use noodles_cram as cram;
/// use noodles_sam as sam;
/// use tokio::io;
///
/// let mut writer = cram::r#async::io::Writer::new(io::sink());
/// writer.write_file_definition().await?;
///
/// let header = sam::Header::default();
/// writer.write_file_header(&header).await?;
///
/// let record = cram::Record::default();
/// writer.write_record(&header, record).await?;
///
/// writer.shutdown(&header).await?;
/// # Ok(())
/// # }
/// ```
pub async fn write_record(
&mut self,
header: &sam::Header,
mut record: Record,
) -> io::Result<()> {
use crate::data_container::builder::AddRecordError;
loop {
match self.data_container_builder.add_record(record) {
Ok(_) => {
self.record_counter += 1;
return Ok(());
}
Err(e) => match e {
AddRecordError::ContainerFull(r) => {
record = r;
self.flush(header).await?;
}
AddRecordError::SliceFull(r) => {
record = r;
}
_ => return Err(io::Error::from(io::ErrorKind::InvalidInput)),
},
}
}
}
/// Writes an alignment record.
///
/// # Examples
///
/// ```
/// # #[tokio::main]
/// # async fn main() -> tokio::io::Result<()> {
/// use noodles_cram as cram;
/// use noodles_sam as sam;
/// use tokio::io;
///
/// let mut writer = cram::r#async::io::Writer::new(io::sink());
/// writer.write_file_definition().await?;
///
/// let header = sam::Header::default();
/// writer.write_file_header(&header).await?;
///
/// let record = sam::Record::default();
/// writer.write_alignment_record(&header, &record).await?;
///
/// writer.shutdown(&header).await?;
/// # Ok(())
/// # }
/// ```
pub async fn write_alignment_record<R>(
&mut self,
header: &sam::Header,
record: &R,
) -> io::Result<()>
where
R: sam::alignment::Record + ?Sized,
{
let record = Record::try_from_alignment_record(header, record)?;
self.write_record(header, record).await
}
async fn flush(&mut self, header: &sam::Header) -> io::Result<()> {
use self::data_container::write_data_container;
if self.data_container_builder.is_empty() {
return Ok(());
}
let data_container_builder = mem::replace(
&mut self.data_container_builder,
DataContainer::builder(self.record_counter),
);
let base_count = data_container_builder.base_count();
let data_container = data_container_builder.build(
&self.options,
&self.reference_sequence_repository,
header,
)?;
write_data_container(&mut self.inner, &data_container, base_count).await
}
}
async fn write_file_definition<W>(
writer: &mut W,
file_definition: &FileDefinition,
) -> io::Result<()>
where
W: AsyncWrite + Unpin,
{
writer.write_all(MAGIC_NUMBER).await?;
write_format(writer, file_definition.version()).await?;
writer.write_all(file_definition.file_id()).await?;
Ok(())
}
async fn write_format<W>(writer: &mut W, version: Version) -> io::Result<()>
where
W: AsyncWrite + Unpin,
{
let format = [version.major(), version.minor()];
writer.write_all(&format).await
}