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//! FASTA reader and iterators.
mod builder;
mod records;
pub mod sequence;
pub use self::{builder::Builder, records::Records};
use std::{
io::{self, BufRead, Seek, SeekFrom},
ops::Range,
};
use noodles_core::{region::Interval, Region};
use crate::{fai, Record};
pub(crate) const DEFINITION_PREFIX: u8 = b'>';
/// A FASTA reader.
pub struct Reader<R> {
inner: R,
}
impl<R> Reader<R>
where
R: BufRead,
{
/// Creates a FASTA reader.
///
/// # Examples
///
/// ```
/// use noodles_fasta as fasta;
/// let data = b">sq0\nACGT\n>sq1\nNNNN\nNNNN\nNN\n";
/// let mut reader = fasta::io::Reader::new(&data[..]);
/// ```
pub fn new(inner: R) -> Self {
Self { inner }
}
/// Returns a reference to the underlying reader.
///
/// # Examples
///
/// ```
/// use noodles_fasta as fasta;
/// let reader = fasta::io::Reader::new(&[][..]);
/// assert!(reader.get_ref().is_empty());
/// ```
pub fn get_ref(&self) -> &R {
&self.inner
}
/// Returns a mutable reference to the underlying reader.
///
/// # Examples
///
/// ```
/// use noodles_fasta as fasta;
/// let mut reader = fasta::io::Reader::new(&[][..]);
/// assert!(reader.get_mut().is_empty());
/// ```
pub fn get_mut(&mut self) -> &mut R {
&mut self.inner
}
/// Returns the underlying reader.
///
/// # Examples
///
/// ```
/// use noodles_fasta as fasta;
/// let reader = fasta::io::Reader::new(&[][..]);
/// assert!(reader.into_inner().is_empty());
/// ```
pub fn into_inner(self) -> R {
self.inner
}
/// Reads a raw definition line.
///
/// The given buffer will not include the trailing newline. It can subsequently be parsed as a
/// [`crate::record::Definition`].
///
/// The position of the stream is expected to be at the start or at the start of another
/// definition.
///
/// If successful, this returns the number of bytes read from the stream. If the number of
/// bytes read is 0, the stream reached EOF.
///
/// # Examples
///
/// ```
/// # use std::io;
/// use noodles_fasta as fasta;
///
/// let data = b">sq0\nACGT\n>sq1\nNNNN\nNNNN\nNN\n";
/// let mut reader = fasta::io::Reader::new(&data[..]);
///
/// let mut buf = String::new();
/// reader.read_definition(&mut buf)?;
///
/// assert_eq!(buf, ">sq0");
/// # Ok::<(), io::Error>(())
/// ```
pub fn read_definition(&mut self, buf: &mut String) -> io::Result<usize> {
read_line(&mut self.inner, buf)
}
/// Reads a sequence.
///
/// The given buffer consumes a sequence without newlines until another definition or EOF is
/// reached.
///
/// The position of the stream is expected to be at the start of a sequence, which is directly
/// after a definition.
///
/// If successful, this returns the number of bases read from the stream. If the number of
/// bases read is 0, the stream reached EOF (though this case is likely an error).
///
/// # Examples
///
/// ```
/// # use std::io;
/// use noodles_fasta as fasta;
///
/// let data = b">sq0\nACGT\n>sq1\nNNNN\nNNNN\nNN\n";
/// let mut reader = fasta::io::Reader::new(&data[..]);
/// reader.read_definition(&mut String::new())?;
///
/// let mut buf = Vec::new();
/// reader.read_sequence(&mut buf)?;
///
/// assert_eq!(buf, b"ACGT");
/// # Ok::<(), io::Error>(())
/// ```
pub fn read_sequence(&mut self, buf: &mut Vec<u8>) -> io::Result<usize> {
use self::sequence::read_sequence;
read_sequence(&mut self.inner, buf)
}
/// Returns a sequence reader.
///
/// A [`sequence::Reader`] can be used for lower-level reading of the raw sequence.
///
/// The position of the stream is expected to be at the start of a sequence to read a full
/// sequence or within a sequence to read a partial one.
///
/// # Examples
///
/// ```
/// # use std::io::{self, Read};
/// use noodles_fasta as fasta;
///
/// let data = b">sq0\nACGT\n>sq1\nNNNN\nNNNN\nNN\n";
/// let mut reader = fasta::io::Reader::new(&data[..]);
/// reader.read_definition(&mut String::new())?;
///
/// let mut sequence_reader = reader.sequence_reader();
/// let mut buf = vec![0; 2];
/// sequence_reader.read_exact(&mut buf)?;
///
/// assert_eq!(buf, b"AC");
/// # Ok::<(), io::Error>(())
/// ```
pub fn sequence_reader(&mut self) -> sequence::Reader<'_, R> {
sequence::Reader::new(self.get_mut())
}
/// Returns an iterator over records starting from the current stream position.
///
/// The position of the stream is expected to be at the start or at the start of another
/// definition.
///
/// ```
/// # use std::io;
/// use noodles_fasta::{self as fasta, record::{Definition, Sequence}};
///
/// let data = b">sq0\nACGT\n>sq1\nNNNN\nNNNN\nNN\n";
/// let mut reader = fasta::io::Reader::new(&data[..]);
///
/// let mut records = reader.records();
///
/// assert_eq!(records.next().transpose()?, Some(fasta::Record::new(
/// Definition::new("sq0", None),
/// Sequence::from(b"ACGT".to_vec()),
/// )));
///
/// assert_eq!(records.next().transpose()?, Some(fasta::Record::new(
/// Definition::new("sq1", None),
/// Sequence::from(b"NNNNNNNNNN".to_vec()),
/// )));
///
/// assert!(records.next().is_none());
/// # Ok::<(), io::Error>(())
/// ```
pub fn records(&mut self) -> Records<'_, R> {
Records::new(self)
}
}
impl<R> Reader<R>
where
R: BufRead + Seek,
{
/// Returns a record of the given region.
///
/// # Examples
///
/// ```
/// # use std::io::Cursor;
/// use noodles_core::Region;
/// use noodles_fasta::{self as fasta, fai, record::{Definition, Sequence}};
///
/// let data = b">sq0\nNNNN\n>sq1\nACGT\n>sq2\nNNNN\n";
/// let index = vec![
/// fai::Record::new("sq0", 4, 5, 4, 5),
/// fai::Record::new("sq1", 4, 15, 4, 5),
/// fai::Record::new("sq2", 4, 25, 4, 5),
/// ];
///
/// let mut reader = fasta::io::Reader::new(Cursor::new(data));
///
/// let region = Region::new("sq1", ..);
/// let record = reader.query(&index, ®ion)?;
/// assert_eq!(record, fasta::Record::new(
/// Definition::new("sq1", None),
/// Sequence::from(b"ACGT".to_vec()),
/// ));
///
/// let region = "sq1:2-3".parse()?;
/// let record = reader.query(&index, ®ion)?;
/// assert_eq!(record, fasta::Record::new(
/// Definition::new("sq1:2-3", None),
/// Sequence::from(b"CG".to_vec()),
/// ));
/// # Ok::<(), Box<dyn std::error::Error>>(())
/// ```
pub fn query(&mut self, index: &fai::Index, region: &Region) -> io::Result<Record> {
use self::sequence::read_sequence_limit;
use crate::record::{Definition, Sequence};
let index_record = index
.iter()
.find(|record| record.name() == region.name())
.ok_or_else(|| {
io::Error::new(
io::ErrorKind::InvalidInput,
format!(
"invalid reference sequence name: {}",
String::from_utf8_lossy(region.name())
),
)
})?;
let seq_len = index_record.length() as usize;
let range = interval_to_slice_range(region.interval(), seq_len);
let pos = index_record.offset()
+ range.start as u64 / index_record.line_bases() * index_record.line_width()
+ range.start as u64 % index_record.line_bases();
self.get_mut().seek(SeekFrom::Start(pos))?;
let definition = Definition::new(region.to_string(), None);
let mut raw_sequence = Vec::new();
read_sequence_limit(&mut self.inner, range.len(), &mut raw_sequence)?;
let sequence = Sequence::from(raw_sequence);
Ok(Record::new(definition, sequence))
}
}
// Reads all bytes until a line feed ('\n') or EOF is reached.
//
// The buffer will not include the trailing newline ('\n' or '\r\n').
pub(crate) fn read_line<R>(reader: &mut R, buf: &mut String) -> io::Result<usize>
where
R: BufRead,
{
const LINE_FEED: char = '\n';
const CARRIAGE_RETURN: char = '\r';
match reader.read_line(buf) {
Ok(0) => Ok(0),
Ok(n) => {
if buf.ends_with(LINE_FEED) {
buf.pop();
if buf.ends_with(CARRIAGE_RETURN) {
buf.pop();
}
}
Ok(n)
}
Err(e) => Err(e),
}
}
// Shifts a 1-based interval to a 0-based range for slicing.
fn interval_to_slice_range<I>(interval: I, len: usize) -> Range<usize>
where
I: Into<Interval>,
{
let interval = interval.into();
let start = interval
.start()
.map(|position| usize::from(position) - 1)
.unwrap_or(usize::MIN);
let end = interval.end().map(usize::from).unwrap_or(len);
start..end
}
#[cfg(test)]
mod tests {
use super::*;
#[test]
fn test_read_definition() -> io::Result<()> {
let data = b">sq0\nACGT\n";
let mut reader = Reader::new(&data[..]);
let mut description_buf = String::new();
reader.read_definition(&mut description_buf)?;
assert_eq!(description_buf, ">sq0");
Ok(())
}
#[test]
fn test_read_line() -> io::Result<()> {
let mut buf = String::new();
let data = b"noodles\n";
let mut reader = &data[..];
buf.clear();
read_line(&mut reader, &mut buf)?;
assert_eq!(buf, "noodles");
let data = b"noodles\r\n";
let mut reader = &data[..];
buf.clear();
read_line(&mut reader, &mut buf)?;
assert_eq!(buf, "noodles");
let data = b"noodles";
let mut reader = &data[..];
buf.clear();
read_line(&mut reader, &mut buf)?;
assert_eq!(buf, "noodles");
Ok(())
}
#[test]
fn test_interval_to_slice_range() -> Result<(), noodles_core::position::TryFromIntError> {
use noodles_core::Position;
const LENGTH: usize = 21;
let start = Position::try_from(8)?;
let end = Position::try_from(13)?;
assert_eq!(interval_to_slice_range(start..=end, LENGTH), 7..13);
assert_eq!(interval_to_slice_range(start.., LENGTH), 7..21);
assert_eq!(interval_to_slice_range(..=end, LENGTH), 0..13);
assert_eq!(interval_to_slice_range(.., LENGTH), 0..21);
Ok(())
}
}