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//! SAM reader.
mod builder;
mod header;
mod query;
mod record;
pub(crate) mod record_buf;
mod record_bufs;
use std::{
io::{self, BufRead},
iter,
};
use noodles_bgzf as bgzf;
use noodles_core::Region;
use noodles_csi::BinningIndex;
pub(crate) use self::record::read_record;
pub use self::{builder::Builder, record_bufs::RecordBufs};
use self::{header::read_header, record_buf::read_record_buf};
use crate::{alignment::RecordBuf, header::ReferenceSequences, Header, Record};
/// A SAM reader.
///
/// The SAM format is comprised of two parts: 1) a header and 2) a list of records.
///
/// Each header line is prefixed with an `@` (at sign). The header is optional and may be empty.
///
/// SAM records are line-based and follow directly after the header or the start of the file until
/// EOF.
///
/// # Examples
///
/// ```no_run
/// use noodles_sam as sam;
///
/// let mut reader = sam::io::reader::Builder::default().build_from_path("sample.sam")?;
/// let header = reader.read_header()?;
///
/// for result in reader.records() {
/// let record = result?;
/// // ...
/// }
/// # Ok::<_, std::io::Error>(())
/// ```
#[derive(Debug)]
pub struct Reader<R> {
inner: R,
buf: Vec<u8>,
}
impl<R> Reader<R> {
/// Returns a reference to the underlying reader.
///
/// # Examples
///
/// ```
/// use noodles_sam as sam;
/// let data = [];
/// let reader = sam::io::Reader::new(&data[..]);
/// assert!(reader.get_ref().is_empty());
/// ```
pub fn get_ref(&self) -> &R {
&self.inner
}
/// Returns a mutable reference to the underlying reader.
///
/// # Examples
///
/// ```
/// use noodles_sam as sam;
/// let data = [];
/// let mut reader = sam::io::Reader::new(&data[..]);
/// assert!(reader.get_mut().is_empty());
/// ```
pub fn get_mut(&mut self) -> &mut R {
&mut self.inner
}
/// Returns the underlying reader.
///
/// # Examples
///
/// ```
/// use noodles_sam as sam;
/// let data = [];
/// let reader = sam::io::Reader::new(&data[..]);
/// assert!(reader.into_inner().is_empty());
/// ```
pub fn into_inner(self) -> R {
self.inner
}
}
impl<R> Reader<R>
where
R: BufRead,
{
/// Creates a SAM reader.
///
/// # Examples
///
/// ```
/// use noodles_sam as sam;
///
/// let data = b"@HD\tVN:1.6
/// *\t4\t*\t0\t255\t*\t*\t0\t0\t*\t*
/// ";
///
/// let reader = sam::io::Reader::new(&data[..]);
/// ```
pub fn new(inner: R) -> Self {
Self::from(inner)
}
/// Reads the SAM header.
///
/// The position of the stream is expected to be at the start.
///
/// The SAM header is optional, and if it is missing, an empty [`Header`] is returned.
///
/// # Examples
///
/// ```
/// # use std::io;
/// use noodles_sam as sam;
///
/// let data = b"@HD\tVN:1.6
/// *\t4\t*\t0\t255\t*\t*\t0\t0\t*\t*
/// ";
///
/// let mut reader = sam::io::Reader::new(&data[..]);
/// let actual = reader.read_header()?;
///
/// let expected = sam::Header::builder()
/// .set_header(Default::default())
/// .build();
///
/// assert_eq!(actual, expected);
/// # Ok::<(), io::Error>(())
/// ```
pub fn read_header(&mut self) -> io::Result<Header> {
read_header(&mut self.inner)
}
/// Reads a record into an alignment record buffer.
///
/// This reads a line from the underlying stream until a newline is reached and parses that
/// line into the given record.
///
/// The stream is expected to be directly after the header or at the start of another record.
///
/// It is more ergonomic to read records using an iterator (see [`Self::records`] and
/// [`Self::query`]), but using this method directly allows reuse of a [`RecordBuf`].
///
/// If successful, the number of bytes read is returned. If the number of bytes read is 0, the
/// stream reached EOF.
///
/// # Examples
///
/// ```
/// use noodles_sam::{self as sam, alignment::RecordBuf};
///
/// let data = b"@HD\tVN:1.6
/// *\t4\t*\t0\t255\t*\t*\t0\t0\t*\t*
/// ";
///
/// let mut reader = sam::io::Reader::new(&data[..]);
/// let header = reader.read_header()?;
///
/// let mut record = RecordBuf::default();
/// reader.read_record_buf(&header, &mut record)?;
///
/// assert_eq!(record, RecordBuf::default());
/// # Ok::<_, std::io::Error>(())
/// ```
pub fn read_record_buf(
&mut self,
header: &Header,
record: &mut RecordBuf,
) -> io::Result<usize> {
read_record_buf(&mut self.inner, &mut self.buf, header, record)
}
/// Returns an iterator over alignment record buffers starting from the current stream
/// position.
///
/// The stream is expected to be directly after the header or at the start of another record.
///
/// # Examples
///
/// ```
/// use noodles_sam as sam;
///
/// let data = b"@HD\tVN:1.6
/// *\t4\t*\t0\t255\t*\t*\t0\t0\t*\t*
/// ";
///
/// let mut reader = sam::io::Reader::new(&data[..]);
/// let header = reader.read_header()?;
///
/// let mut records = reader.record_bufs(&header);
/// assert!(records.next().is_some());
/// assert!(records.next().is_none());
/// # Ok::<_, std::io::Error>(())
/// ```
pub fn record_bufs<'a>(&'a mut self, header: &'a Header) -> RecordBufs<'a, R> {
RecordBufs::new(self, header)
}
/// Reads a record.
///
/// This reads SAM fields from the underlying stream into the given record's buffer until a
/// newline is reached. No fields are decoded, meaning the record is not necessarily valid.
/// However, the structure of the buffer is guaranteed to be record-like.
///
/// The stream is expected to be directly after the header or at the start of another record.
///
/// If successful, the number of bytes read is returned. If the number of bytes read is 0, the
/// stream reached EOF.
///
/// # Examples
///
/// ```
/// # use std::io;
/// use noodles_sam as sam;
///
/// let data = b"@HD\tVN:1.6
/// *\t4\t*\t0\t255\t*\t*\t0\t0\t*\t*
/// ";
///
/// let mut reader = sam::io::Reader::new(&data[..]);
/// reader.read_header()?;
///
/// let mut record = sam::Record::default();
/// reader.read_record(&mut record)?;
/// # Ok::<(), io::Error>(())
/// ```
pub fn read_record(&mut self, record: &mut Record) -> io::Result<usize> {
read_record(&mut self.inner, record)
}
/// Returns an iterator over records.
///
/// The stream is expected to be directly after the reference sequences or at the start of
/// another record.
///
/// # Examples
///
/// ```
/// use noodles_sam as sam;
///
/// let data = b"@HD\tVN:1.6
/// *\t4\t*\t0\t255\t*\t*\t0\t0\t*\t*
/// ";
///
/// let mut reader = sam::io::Reader::new(&data[..]);
/// reader.read_header()?;
///
/// for result in reader.records() {
/// let record = result?;
/// // ...
/// }
/// # Ok::<_, std::io::Error>(())
/// ```
pub fn records(&mut self) -> impl Iterator<Item = io::Result<Record>> + '_ {
let mut record = Record::default();
iter::from_fn(move || match self.read_record(&mut record) {
Ok(0) => None,
Ok(_) => Some(Ok(record.clone())),
Err(e) => Some(Err(e)),
})
}
}
impl<R> Reader<R>
where
R: bgzf::io::BufRead + bgzf::io::Seek,
{
// Seeks to the first record by setting the cursor to the beginning of the stream and
// (re)reading the header.
fn seek_to_first_record(&mut self) -> io::Result<bgzf::VirtualPosition> {
self.get_mut()
.seek_to_virtual_position(bgzf::VirtualPosition::default())?;
self.read_header()?;
Ok(self.get_ref().virtual_position())
}
/// Returns an iterator over records that intersect the given region.
///
/// To query for unmapped records, use [`Self::query_unmapped`].
///
/// # Examples
///
/// ```no_run
/// # use std::{fs::File, io};
/// use noodles_bgzf as bgzf;
/// use noodles_csi as csi;
/// use noodles_sam as sam;
///
/// let mut reader = File::open("sample.sam.gz")
/// .map(bgzf::Reader::new)
/// .map(sam::io::Reader::new)?;
///
/// let header = reader.read_header()?;
///
/// let index = csi::read("sample.sam.gz.csi")?;
/// let region = "sq0:8-13".parse()?;
/// let query = reader.query(&header, &index, ®ion)?;
///
/// for result in query {
/// let record = result?;
/// // ...
/// }
/// # Ok::<(), Box<dyn std::error::Error>>(())
/// ```
pub fn query<'a, I>(
&'a mut self,
header: &'a Header,
index: &I,
region: &Region,
) -> io::Result<impl Iterator<Item = io::Result<Record>> + 'a>
where
I: BinningIndex,
{
use self::query::{FilterByRegion, Query};
let reference_sequence_id = resolve_region(header.reference_sequences(), region)?;
let chunks = index.query(reference_sequence_id, region.interval())?;
Ok(FilterByRegion::new(
Query::new(self.get_mut(), chunks),
header,
reference_sequence_id,
region.interval(),
))
}
/// Returns an iterator of unmapped records after querying for the unmapped region.
///
/// ```no_run
/// # use std::{fs::File, io};
/// use noodles_bgzf as bgzf;
/// use noodles_csi as csi;
/// use noodles_sam as sam;
///
/// let mut reader = File::open("sample.sam.gz")
/// .map(bgzf::Reader::new)
/// .map(sam::io::Reader::new)?;
///
/// reader.read_header()?;
///
/// let index = csi::read("sample.sam.gz.csi")?;
/// let query = reader.query_unmapped(&index)?;
///
/// for result in query {
/// let record = result?;
/// // ...
/// }
/// # Ok::<_, io::Error>(())
/// ```
pub fn query_unmapped<I>(
&mut self,
index: &I,
) -> io::Result<impl Iterator<Item = io::Result<Record>> + '_>
where
I: BinningIndex,
{
if let Some(pos) = index.last_first_record_start_position() {
self.get_mut().seek_to_virtual_position(pos)?;
} else {
self.seek_to_first_record()?;
}
let mut record = Record::default();
Ok(iter::from_fn(move || loop {
match self.read_record(&mut record) {
Ok(0) => return None,
Ok(_) => {
let result = record.flags().map(|flags| flags.is_unmapped());
match result {
Ok(true) => return Some(Ok(record.clone())),
Ok(false) => {}
Err(e) => return Some(Err(e)),
}
}
Err(e) => return Some(Err(e)),
}
}))
}
}
impl<R> From<R> for Reader<R>
where
R: BufRead,
{
fn from(inner: R) -> Self {
Self {
inner,
buf: Vec::new(),
}
}
}
impl<R> crate::alignment::io::Read<R> for Reader<R>
where
R: BufRead,
{
fn read_alignment_header(&mut self) -> io::Result<Header> {
self.read_header()
}
fn alignment_records<'a>(
&'a mut self,
header: &'a Header,
) -> Box<dyn Iterator<Item = io::Result<Box<dyn crate::alignment::Record>>> + 'a> {
Box::new(self.record_bufs(header).map(|result| {
result.map(|record| Box::new(record) as Box<dyn crate::alignment::Record>)
}))
}
}
fn read_line<R>(reader: &mut R, buf: &mut Vec<u8>) -> io::Result<usize>
where
R: BufRead,
{
const LINE_FEED: u8 = b'\n';
const CARRIAGE_RETURN: u8 = b'\r';
match reader.read_until(LINE_FEED, buf)? {
0 => Ok(0),
n => {
if buf.ends_with(&[LINE_FEED]) {
buf.pop();
if buf.ends_with(&[CARRIAGE_RETURN]) {
buf.pop();
}
}
Ok(n)
}
}
}
fn resolve_region(reference_sequences: &ReferenceSequences, region: &Region) -> io::Result<usize> {
reference_sequences
.get_index_of(region.name())
.ok_or_else(|| {
io::Error::new(
io::ErrorKind::InvalidInput,
format!(
"region reference sequence does not exist in reference sequences: {region:?}"
),
)
})
}
#[cfg(test)]
mod tests {
use super::*;
#[test]
fn test_read_line() -> io::Result<()> {
fn t(buf: &mut Vec<u8>, mut reader: &[u8], expected: &[u8]) -> io::Result<()> {
buf.clear();
read_line(&mut reader, buf)?;
assert_eq!(buf, expected);
Ok(())
}
let mut buf = Vec::new();
t(&mut buf, b"noodles\n", b"noodles")?;
t(&mut buf, b"noodles\r\n", b"noodles")?;
t(&mut buf, b"noodles", b"noodles")?;
Ok(())
}
}