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pub mod alternate_bases;
pub mod builder;
pub mod chromosome;
mod field;
pub mod filters;
pub mod format;
pub mod genotype;
pub mod ids;
pub mod info;
pub mod position;
pub mod quality_score;
pub mod reference_bases;
pub(crate) mod value;
pub use self::{
alternate_bases::AlternateBases, builder::Builder, chromosome::Chromosome, field::Field,
filters::Filters, format::Format, genotype::Genotype, ids::Ids, info::Info, position::Position,
quality_score::QualityScore, reference_bases::ReferenceBases,
};
use std::{convert::TryFrom, error, fmt, num, str::FromStr};
pub(crate) const MISSING_FIELD: &str = ".";
pub(crate) const FIELD_DELIMITER: char = '\t';
#[derive(Clone, Debug, PartialEq)]
pub struct Record {
chromosome: Chromosome,
position: Position,
ids: Ids,
reference_bases: ReferenceBases,
alternate_bases: AlternateBases,
quality_score: QualityScore,
filters: Filters,
info: Info,
format: Option<Format>,
genotypes: Vec<Genotype>,
}
impl Record {
pub fn builder() -> Builder {
Builder::default()
}
pub fn chromosome(&self) -> &Chromosome {
&self.chromosome
}
pub fn position(&self) -> Position {
self.position
}
pub fn ids(&self) -> &Ids {
&self.ids
}
pub fn reference_bases(&self) -> &ReferenceBases {
&self.reference_bases
}
pub fn alternate_bases(&self) -> &AlternateBases {
&self.alternate_bases
}
pub fn quality_score(&self) -> QualityScore {
self.quality_score
}
pub fn filters(&self) -> &Filters {
&self.filters
}
pub fn info(&self) -> &Info {
&self.info
}
pub fn format(&self) -> Option<&Format> {
self.format.as_ref()
}
pub fn genotypes(&self) -> &[Genotype] {
&self.genotypes
}
}
#[derive(Clone, Debug, Eq, PartialEq)]
pub enum EndError {
InvalidPosition(position::TryFromIntError),
InvalidInfoEndPositionFieldValue,
InvalidReferenceBasesLength(num::TryFromIntError),
PositionOverflow(i32, i32),
}
impl error::Error for EndError {}
impl fmt::Display for EndError {
fn fmt(&self, f: &mut fmt::Formatter<'_>) -> fmt::Result {
match self {
Self::InvalidPosition(e) => write!(f, "invalid position: {}", e),
Self::InvalidInfoEndPositionFieldValue => {
write!(f, "invalid INFO end position (`END`) field value type")
}
Self::InvalidReferenceBasesLength(e) => {
write!(f, "invalid reference base length: {}", e)
}
Self::PositionOverflow(start, len) => write!(
f,
"calculation of the end position overflowed: {} + {}",
start, len,
),
}
}
}
impl Record {
pub fn end(&self) -> Result<Position, EndError> {
use info::field::{Key, Value};
let end = if let Some(field) = self.info().get(&Key::EndPosition) {
match field.value() {
Value::Integer(n) => *n,
_ => return Err(EndError::InvalidInfoEndPositionFieldValue),
}
} else {
let start = i32::from(self.position());
let len = i32::try_from(self.reference_bases().len())
.map_err(EndError::InvalidReferenceBasesLength)?;
start
.checked_add(len - 1)
.ok_or(EndError::PositionOverflow(start, len))?
};
Position::try_from(end).map_err(EndError::InvalidPosition)
}
}
impl fmt::Display for Record {
fn fmt(&self, f: &mut fmt::Formatter<'_>) -> fmt::Result {
write!(
f,
"{chrom}\t{pos}\t{id}\t{ref}\t{alt}\t{qual}\t{filter}\t{info}",
chrom = self.chromosome(),
pos = i32::from(self.position()),
id = self.ids(),
r#ref = self.reference_bases(),
alt = self.alternate_bases(),
qual = self.quality_score(),
filter = self.filters(),
info = self.info(),
)?;
if let Some(format) = self.format() {
write!(f, "\t{}", format)?;
for field in self.genotypes() {
write!(f, "\t{}", field)?;
}
}
Ok(())
}
}
#[derive(Clone, Debug, Eq, PartialEq)]
pub enum ParseError {
MissingField(Field),
InvalidChromosome(chromosome::ParseError),
InvalidPosition(position::ParseError),
InvalidIds(ids::ParseError),
InvalidReferenceBases(reference_bases::ParseError),
InvalidAlternateBases(alternate_bases::ParseError),
InvalidQualityScore(quality_score::ParseError),
InvalidFilters(filters::ParseError),
InvalidInfo(info::ParseError),
InvalidFormat(format::ParseError),
InvalidGenotype(genotype::ParseError),
}
impl error::Error for ParseError {}
impl fmt::Display for ParseError {
fn fmt(&self, f: &mut fmt::Formatter<'_>) -> fmt::Result {
match self {
Self::MissingField(field) => write!(f, "missing field: {}", field),
Self::InvalidChromosome(e) => write!(f, "invalid chromosome: {}", e),
Self::InvalidPosition(e) => write!(f, "invalid position: {}", e),
Self::InvalidIds(e) => write!(f, "invalid IDs: {}", e),
Self::InvalidReferenceBases(e) => write!(f, "invalid reference bases: {}", e),
Self::InvalidAlternateBases(e) => write!(f, "invalid alternate bases: {}", e),
Self::InvalidQualityScore(e) => write!(f, "invalid quality score: {}", e),
Self::InvalidFilters(e) => write!(f, "invalid filters: {}", e),
Self::InvalidInfo(e) => write!(f, "invalid info: {}", e),
Self::InvalidFormat(e) => write!(f, "invalid format: {}", e),
Self::InvalidGenotype(e) => write!(f, "invalid genotype: {}", e),
}
}
}
impl FromStr for Record {
type Err = ParseError;
fn from_str(s: &str) -> Result<Self, Self::Err> {
let mut fields = s.split(FIELD_DELIMITER);
let chrom = parse_string(&mut fields, Field::Chromosome)
.and_then(|s| s.parse().map_err(ParseError::InvalidChromosome))?;
let pos = parse_string(&mut fields, Field::Position)
.and_then(|s| s.parse().map_err(ParseError::InvalidPosition))?;
let ids = parse_string(&mut fields, Field::Ids)
.and_then(|s| s.parse().map_err(ParseError::InvalidIds))?;
let r#ref = parse_string(&mut fields, Field::ReferenceBases)
.and_then(|s| s.parse().map_err(ParseError::InvalidReferenceBases))?;
let alt = parse_string(&mut fields, Field::AlternateBases)
.and_then(|s| s.parse().map_err(ParseError::InvalidAlternateBases))?;
let qual = parse_string(&mut fields, Field::QualityScore)
.and_then(|s| s.parse().map_err(ParseError::InvalidQualityScore))?;
let filter = parse_string(&mut fields, Field::Filters)
.and_then(|s| s.parse().map_err(ParseError::InvalidFilters))?;
let info = parse_string(&mut fields, Field::Info)
.and_then(|s| s.parse().map_err(ParseError::InvalidInfo))?;
let format = match fields.next() {
Some(s) => s.parse().map(Some).map_err(ParseError::InvalidFormat)?,
None => None,
};
let genotypes = format
.as_ref()
.map(|f| {
fields
.map(|s| Genotype::from_str_format(s, f))
.collect::<Result<_, _>>()
.map_err(ParseError::InvalidGenotype)
})
.unwrap_or_else(|| Ok(Vec::new()))?;
Ok(Self {
chromosome: chrom,
position: pos,
ids,
reference_bases: r#ref,
alternate_bases: alt,
quality_score: qual,
filters: filter,
info,
format,
genotypes,
})
}
}
fn parse_string<'a, I>(fields: &mut I, field: Field) -> Result<&'a str, ParseError>
where
I: Iterator<Item = &'a str>,
{
fields.next().ok_or(ParseError::MissingField(field))
}
#[cfg(test)]
mod tests {
use std::convert::TryFrom;
use super::*;
#[test]
fn test_end() -> Result<(), Box<dyn std::error::Error>> {
let record = Record::builder()
.set_chromosome("sq0".parse()?)
.set_position(Position::try_from(1)?)
.set_reference_bases("A".parse()?)
.set_info(Info::try_from(vec![info::Field::new(
info::field::Key::EndPosition,
info::field::Value::Flag,
)])?)
.build()?;
assert_eq!(
record.end(),
Err(EndError::InvalidInfoEndPositionFieldValue)
);
let record = Record::builder()
.set_chromosome("sq0".parse()?)
.set_position(Position::try_from(i32::MAX)?)
.set_reference_bases("ACGT".parse()?)
.build()?;
assert_eq!(record.end(), Err(EndError::PositionOverflow(i32::MAX, 4)));
Ok(())
}
#[test]
fn test_fmt() -> Result<(), Box<dyn std::error::Error>> {
let record = Record::builder()
.set_chromosome("sq0".parse()?)
.set_position(Position::try_from(1)?)
.set_reference_bases("A".parse()?)
.build()?;
assert_eq!(record.to_string(), "sq0\t1\t.\tA\t.\t.\t.\t.");
Ok(())
}
#[test]
fn test_fmt_with_format() -> Result<(), Box<dyn std::error::Error>> {
let format: Format = "GT:GQ".parse()?;
let record = Record::builder()
.set_chromosome("sq0".parse()?)
.set_position(Position::try_from(1)?)
.set_reference_bases("A".parse()?)
.set_format(format.clone())
.add_genotype(Genotype::from_str_format("0|0:13", &format)?)
.build()?;
assert_eq!(
record.to_string(),
"sq0\t1\t.\tA\t.\t.\t.\t.\tGT:GQ\t0|0:13"
);
Ok(())
}
#[test]
fn test_from_str() -> Result<(), ParseError> {
use alternate_bases::Allele;
use reference_bases::Base;
let s = "chr1\t13\tnd0\tATCG\tA\t5.8\tPASS\tSVTYPE=DEL";
let record: Record = s.parse()?;
assert!(matches!(record.chromosome(), Chromosome::Name(name) if name == "chr1"));
assert_eq!(i32::from(record.position()), 13);
let ids = record.ids();
assert_eq!(ids.len(), 1);
assert!(ids.contains("nd0"));
let reference_bases = [Base::A, Base::T, Base::C, Base::G];
assert_eq!(&record.reference_bases()[..], &reference_bases[..]);
let alternate_bases = [Allele::Bases(vec![Base::A])];
assert_eq!(&record.alternate_bases()[..], &alternate_bases[..]);
assert_eq!(*record.quality_score(), Some(5.8));
assert_eq!(record.filters(), &Filters::Pass);
assert_eq!(record.info().len(), 1);
assert!(record.format().is_none());
assert!(record.genotypes().is_empty());
Ok(())
}
#[test]
fn test_from_str_with_genotype_info() -> Result<(), Box<dyn std::error::Error>> {
let s = "chr1\t13\tnd0\tATCG\tA\t5.8\tPASS\tSVTYPE=DEL\tGT:GQ\t0|1:13";
let record: Record = s.parse()?;
let expected = Format::try_from(vec![
genotype::field::Key::Genotype,
genotype::field::Key::ConditionalGenotypeQuality,
])?;
assert_eq!(record.format(), Some(&expected));
let genotypes = record.genotypes();
assert_eq!(genotypes.len(), 1);
let actual: Vec<_> = genotypes[0].values().cloned().collect();
let expected = vec![
genotype::Field::new(
genotype::field::Key::Genotype,
Some(genotype::field::Value::String(String::from("0|1"))),
),
genotype::Field::new(
genotype::field::Key::ConditionalGenotypeQuality,
Some(genotype::field::Value::Integer(13)),
),
];
assert_eq!(actual, expected);
Ok(())
}
}