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//! VCF reader and iterators.
mod builder;
mod header;
pub(crate) mod query;
pub(crate) mod record;
pub mod record_buf;
mod record_bufs;
use self::record::read_record;
pub(crate) use self::record_buf::parse_record_buf;
pub use self::{builder::Builder, query::Query, record_bufs::RecordBufs};
use std::{
io::{self, BufRead},
iter, str,
};
use noodles_bgzf as bgzf;
use noodles_core::Region;
use noodles_csi::BinningIndex;
use self::header::read_header;
use crate::{variant::RecordBuf, Header, Record};
/// A VCF reader.
///
/// The VCF format has two main parts: 1) a header and 2) a list of VCF records.
///
/// Each header line is prefixed with a `#` (number sign) and is terminated by the header header
/// (`#CHROM`...; inclusive).
///
/// VCF records are line-based and follow directly after the header until EOF.
///
/// # Examples
///
/// ```no_run
/// use noodles_vcf as vcf;
///
/// let mut reader = vcf::io::reader::Builder::default().build_from_path("sample.vcf")?;
/// let header = reader.read_header()?;
///
/// for result in reader.records() {
/// let record = result?;
/// // ...
/// }
/// # Ok::<_, std::io::Error>(())
/// ```
#[derive(Debug)]
pub struct Reader<R> {
inner: R,
buf: String,
}
impl<R> Reader<R> {
/// Returns a reference to the underlying reader.
///
/// # Examples
///
/// ```
/// use noodles_vcf as vcf;
/// let data = [];
/// let reader = vcf::io::Reader::new(&data[..]);
/// assert!(reader.get_ref().is_empty());
/// ```
pub fn get_ref(&self) -> &R {
&self.inner
}
/// Returns a mutable reference to the underlying reader.
///
/// # Examples
///
/// ```
/// use noodles_vcf as vcf;
/// let data = [];
/// let mut reader = vcf::io::Reader::new(&data[..]);
/// assert!(reader.get_mut().is_empty());
/// ```
pub fn get_mut(&mut self) -> &mut R {
&mut self.inner
}
/// Unwraps and returns the underlying writer.
///
/// # Examples
///
/// ```
/// use noodles_vcf as vcf;
/// let data = [];
/// let reader = vcf::io::Reader::new(&data[..]);
/// assert!(reader.into_inner().is_empty());
/// ```
pub fn into_inner(self) -> R {
self.inner
}
}
impl<R> Reader<R>
where
R: BufRead,
{
/// Creates a VCF reader.
///
/// # Examples
///
/// ```
/// use noodles_vcf as vcf;
///
/// let data = b"##fileformat=VCFv4.3
/// #CHROM\tPOS\tID\tREF\tALT\tQUAL\tFILTER\tINFO
/// sq0\t1\t.\tA\t.\t.\tPASS\t.
/// ";
///
/// let reader = vcf::io::Reader::new(&data[..]);
/// ```
pub fn new(inner: R) -> Self {
Self {
inner,
buf: String::new(),
}
}
/// Reads the VCF header.
///
/// This reads all header lines prefixed with a `#` (number sign), which includes the header
/// header (`#CHROM`...), and parses it as a [`crate::Header`].
///
/// The position of the stream is expected to be at the start.
///
/// # Examples
///
/// ```
/// # use std::io;
/// use noodles_vcf as vcf;
///
/// let data = b"##fileformat=VCFv4.3
/// #CHROM\tPOS\tID\tREF\tALT\tQUAL\tFILTER\tINFO
/// sq0\t1\t.\tA\t.\t.\tPASS\t.
/// ";
///
/// let mut reader = vcf::io::Reader::new(&data[..]);
/// let header = reader.read_header()?;
/// # Ok::<(), io::Error>(())
/// ```
pub fn read_header(&mut self) -> io::Result<Header> {
read_header(&mut self.inner)
}
/// Reads a single VCF record.
///
/// This reads a line from the underlying stream until a newline is reached and parses that
/// line into the given record.
///
/// The stream is expected to be directly after the header or at the start of another record.
///
/// It is more ergonomic to read records using an iterator (see [`Self::records`]), but using
/// this method allows control of the record buffer.
///
/// If successful, the number of bytes read is returned. If the number of bytes read is 0, the
/// stream reached EOF.
///
/// # Examples
///
/// ```
/// use noodles_vcf as vcf;
///
/// let data = b"##fileformat=VCFv4.3
/// #CHROM\tPOS\tID\tREF\tALT\tQUAL\tFILTER\tINFO
/// sq0\t1\t.\tA\t.\t.\tPASS\t.
/// ";
///
/// let mut reader = vcf::io::Reader::new(&data[..]);
/// let header = reader.read_header()?;
///
/// let mut record = vcf::variant::RecordBuf::default();
/// reader.read_record_buf(&header, &mut record)?;
/// # Ok::<_, std::io::Error>(())
/// ```
pub fn read_record_buf(
&mut self,
header: &Header,
record: &mut RecordBuf,
) -> io::Result<usize> {
self.buf.clear();
match read_line(&mut self.inner, &mut self.buf)? {
0 => Ok(0),
n => {
parse_record_buf(&self.buf, header, record)
.map_err(|e| io::Error::new(io::ErrorKind::InvalidData, e))?;
Ok(n)
}
}
}
/// Returns an iterator over records starting from the current stream position.
///
/// The stream is expected to be directly after the header or at the start of another record.
///
/// # Examples
///
/// ```
/// use noodles_vcf as vcf;
///
/// let data = b"##fileformat=VCFv4.3
/// #CHROM\tPOS\tID\tREF\tALT\tQUAL\tFILTER\tINFO
/// sq0\t1\t.\tA\t.\t.\tPASS\t.
/// ";
///
/// let mut reader = vcf::io::Reader::new(&data[..]);
/// let header = reader.read_header()?;
///
/// let mut records = reader.record_bufs(&header);
/// assert!(records.next().is_some());
/// assert!(records.next().is_none());
/// # Ok::<_, std::io::Error>(())
/// ```
pub fn record_bufs<'r, 'h: 'r>(&'r mut self, header: &'h Header) -> RecordBufs<'r, 'h, R> {
RecordBufs::new(self, header)
}
/// Reads a single record without eagerly parsing its fields.
///
/// The reads VCF record fields from the underlying stream into the given record's buffer until
/// a newline is reached. No fields are parsed, meaning the record is not necessarily valid.
/// However, the structure of the line is guaranteed to be record-like.
///
/// The stream is expected to be directly after the header or at the start of another record.
///
/// If successful, the number of bytes read is returned. If the number of bytes read is 0, the
/// stream reached EOF.
///
/// # Examples
///
/// ```
/// use noodles_vcf as vcf;
///
/// let data = b"##fileformat=VCFv4.3
/// #CHROM\tPOS\tID\tREF\tALT\tQUAL\tFILTER\tINFO
/// sq0\t1\t.\tA\t.\t.\tPASS\t.
/// ";
///
/// let mut reader = vcf::io::Reader::new(&data[..]);
/// reader.read_header()?;
///
/// let mut record = vcf::Record::default();
/// reader.read_record(&mut record)?;
/// # Ok::<_, std::io::Error>(())
/// ```
pub fn read_record(&mut self, record: &mut Record) -> io::Result<usize> {
read_record(&mut self.inner, record)
}
/// Returns an iterator over records.
///
/// The stream is expected to be directly after the header or at the start of another record.
///
/// # Examples
///
/// ```
/// use noodles_vcf as vcf;
///
/// const DATA: &[u8] = b"##fileformat=VCFv4.3
/// #CHROM\tPOS\tID\tREF\tALT\tQUAL\tFILTER\tINFO
/// sq0\t1\t.\tA\t.\t.\tPASS\t.
/// ";
///
/// let mut reader = vcf::io::Reader::new(DATA);
/// reader.read_header()?;
///
/// for result in reader.records() {
/// let record = result?;
/// // ...
/// }
/// # Ok::<_, std::io::Error>(())
/// ```
pub fn records(&mut self) -> impl Iterator<Item = io::Result<Record>> + '_ {
let mut record = Record::default();
iter::from_fn(move || match self.read_record(&mut record) {
Ok(0) => None,
Ok(_) => Some(Ok(record.clone())),
Err(e) => Some(Err(e)),
})
}
}
impl<R> Reader<R>
where
R: bgzf::io::BufRead + bgzf::io::Seek,
{
/// Returns an iterator over records that intersects the given region.
///
/// # Examples
///
/// ```no_run
/// # use std::fs::File;
/// use noodles_bgzf as bgzf;;
/// use noodles_core::Region;
/// use noodles_tabix as tabix;
/// use noodles_vcf as vcf;
///
/// let mut reader = File::open("sample.vcf.gz")
/// .map(bgzf::Reader::new)
/// .map(vcf::io::Reader::new)?;
///
/// let header = reader.read_header()?;
///
/// let index = tabix::read("sample.vcf.gz.tbi")?;
/// let region = "sq0:8-13".parse()?;
/// let query = reader.query(&header, &index, ®ion)?;
///
/// for result in query {
/// let record = result?;
/// println!("{:?}", record);
/// }
/// Ok::<_, Box<dyn std::error::Error>>(())
/// ```
pub fn query<'r, 'h, I>(
&'r mut self,
header: &'h Header,
index: &I,
region: &Region,
) -> io::Result<Query<'r, 'h, R>>
where
I: BinningIndex,
{
let (reference_sequence_id, reference_sequence_name) = resolve_region(index, region)?;
let chunks = index.query(reference_sequence_id, region.interval())?;
Ok(Query::new(
self.get_mut(),
chunks,
reference_sequence_name,
region.interval(),
header,
))
}
}
impl<R> crate::variant::io::Read<R> for Reader<R>
where
R: BufRead,
{
fn read_variant_header(&mut self) -> io::Result<Header> {
self.read_header()
}
fn variant_records<'r, 'h: 'r>(
&'r mut self,
_: &'h Header,
) -> Box<dyn Iterator<Item = io::Result<Box<dyn crate::variant::Record>>> + 'r> {
Box::new(
self.records().map(|result| {
result.map(|record| Box::new(record) as Box<dyn crate::variant::Record>)
}),
)
}
}
// Reads all bytes until a line feed ('\n') or EOF is reached.
//
// The buffer will not include the trailing newline ('\n' or '\r\n').
fn read_line<R>(reader: &mut R, buf: &mut String) -> io::Result<usize>
where
R: BufRead,
{
const LINE_FEED: char = '\n';
const CARRIAGE_RETURN: char = '\r';
match reader.read_line(buf) {
Ok(0) => Ok(0),
Ok(n) => {
if buf.ends_with(LINE_FEED) {
buf.pop();
if buf.ends_with(CARRIAGE_RETURN) {
buf.pop();
}
}
Ok(n)
}
Err(e) => Err(e),
}
}
pub(crate) fn resolve_region<I>(index: &I, region: &Region) -> io::Result<(usize, Vec<u8>)>
where
I: BinningIndex,
{
let header = index
.header()
.ok_or_else(|| io::Error::new(io::ErrorKind::InvalidInput, "missing tabix header"))?;
let region_name = str::from_utf8(region.name())
.map_err(|e| io::Error::new(io::ErrorKind::InvalidInput, e))?;
let i = header
.reference_sequence_names()
.get_index_of(region_name)
.ok_or_else(|| {
io::Error::new(
io::ErrorKind::InvalidInput,
format!(
"region reference sequence does not exist in reference sequences: {region:?}"
),
)
})?;
Ok((i, region.name().to_vec()))
}
#[cfg(test)]
mod tests {
use super::*;
#[test]
fn test_read_record() -> io::Result<()> {
static DATA: &[u8] = b"\
##fileformat=VCFv4.3
##fileDate=20200501
#CHROM\tPOS\tID\tREF\tALT\tQUAL\tFILTER\tINFO
sq0\t1\t.\tA\t.\t.\tPASS\t.
";
let mut reader = Reader::new(DATA);
let header = reader.read_header()?;
let mut record = RecordBuf::default();
let bytes_read = reader.read_record_buf(&header, &mut record)?;
assert_eq!(bytes_read, 21);
let bytes_read = reader.read_record_buf(&header, &mut record)?;
assert_eq!(bytes_read, 0);
Ok(())
}
#[test]
fn test_read_line() -> io::Result<()> {
let mut buf = String::new();
let data = b"noodles\n";
let mut reader = &data[..];
buf.clear();
read_line(&mut reader, &mut buf)?;
assert_eq!(buf, "noodles");
let data = b"noodles\r\n";
let mut reader = &data[..];
buf.clear();
read_line(&mut reader, &mut buf)?;
assert_eq!(buf, "noodles");
let data = b"noodles";
let mut reader = &data[..];
buf.clear();
read_line(&mut reader, &mut buf)?;
assert_eq!(buf, "noodles");
Ok(())
}
}